Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 10
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 102
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شناسه ملی سند علمی:
JR_IJBMS-26-10_013
تاریخ نمایه سازی: 4 شهریور 1402
چکیده مقاله:
Objective(s): Targeting the lytic cycle of the Epstein-Barr virus (EBV) has been considered a new treatment strategy for malignancies caused by this virus. This study aimed to investigate the effect of Dendrosomal NanoCurcumin (DNC) to prevent cell transformation and inhibit the expression of viral lytic gene expression in the generation of lymphoblastoid cell line (LCL).Materials and Methods: Cell viability of LCLs and PBMCs was performed by MTT assay, and flow cytometry (Annexin/PI) was used for evaluation of apoptosis. CD markers on the surface of generated LCL (CD۱۹) cells were examined for cell validation. The effect of DNC on transformation was evaluated by examining cell morphology and determining the expression level of lytic genes BZLF۱, Zta, BHRF۱, and BRLF۱ of EBV using Real-time PCR. Student’s t-test was used for statistical analysis.Results: The MTT assay showed that DNC can inhibit the proliferation of LCL in a dose-dependent manner. The ۵۰% cytotoxic concentration (CC۵۰) of DNC and curcumin for LCL was determined ۳۸.۸ µg/ml and ۷۵ µg/ml, respectively after ۷۲ hr. Also, Real-time PCR data analysis showed that DNC in ۳۰ µg/ml concentration significantly inhibited cell transformation in the LCL and significantly reduced viral lytic genes such as BZLF۱, Zta, BHRF۱, and BRLF۱expression compared to control.Conclusion: Overall, these findings show that DNC reduces the expression of the viral lytic cycle genes and also the induction of cell apoptosis and finally prevents the generation of LCL.
کلیدواژه ها:
نویسندگان
Mahboobeh Cheragh
Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Majid Sadeghizadeh
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Mohammad Hasan Pouriayevali
Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran
Masoud Parsania
Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
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