Antioxidant Effect of Ardeh and Honey Combination on Ethanol-induced Oxidative stress in Liver and Kidney of Rats

Background: The pathogenesis of ethanol in the liver and kidney is associated with an increase in free radicals and oxidative stress that leads to structural and functional abnormalities in these vital organs. Methods: Forty-eight male wistar rats were placed in eight groups and were treated as follows: control group (normal saline), group of ethanol (۴ g/ kg), three doses of Ardeh and honey combination (۱, ۲ and ۵ g/ kg body weight), and the three groups received three doses of Ardeh and honey combination with ethanol (the same three doses of combinations along with ۴ g/ kg ethanol). All treatments were performed once daily for ۶۰ consecutive days with method of stomach gavages. Serum, liver and kidney tissue samples were obtained in order to assess serum components, tissue antioxidant enzyme activities, and the level of thiobarbituric acid reactive substances (TBARS). Results: Hepatic superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in the ethanol group significantly decreased compared with the control group, while the Ardeh and honey combination (۵ g) could only significantly increased glutathione peroxidase activity. Renal GPx activity in ethanol consumption significantly increased compared to controls. GPx activity in Ardeh and honey combination and alcohol (۵ g, ۴ g) were significantly reduced compared to ethanol group. In the present study, the amount of TBARS which represents the rate of lipid peroxidation, in liver of ethanol group compared with the control group showed a significant increase (P<۰.۰۵). Conclusion: Ardeh and honey combination as a pre-treatment, protect liver and kidney against oxidative damage induced by ethanol.