The investigation of circfoxo۳ rs۱۲۱۹۶۹۹۶ polymorphic site in colorectal Cancer

circFOXO۳ is a member of circRNA family, which is closely related to tumor occurrence, cell survival, and apoptosis. Moreover, circ‑FOXO۳ was associated with tumor size, distant metastasis, differentiation, lymph node metastasis and, TMN stages of patients with CRC. circ‑FOXO۳ overexpression suppressed CRC cell proliferation, migration and, invasion. However, the diverse functions of circFOXO۳ have not been fully understood. Single nucleotide polymorphisms (SNPs) are associated with many disease occurrences. We selected a single nucleotide polymorphism rs۱۲۱۹۶۹۹۶ for genotype frequency analysis and assessed the frequency of circFOXO۳ gene polymorphisms in ۲۰ colorectal cancer patients and ۵۰ randomly selected control subject. Blood samples of colorectal cancer cases and healthy individuals were collected. Genomic DNA was extracted from whole blood samples using a commercial DNA extraction kit. DNA was diluted to ۱۰۰ ng/μl with Tris-EDTA (TE) buffer, and then ۱ μl of each sample was used to amplify the polymorphic site. For the genotyping analysis, ARMS PCR will be used. The specific primers were designed using Oligo ۷ software. To estimate the relative colorectal cancer risk of the various genotypes, the odds ratio (OR) and ۹۵% CI will be calculated. The statistical significance of differences between the colorectal and control groups will be determined by the Student’s t-test for continuous variables and the χ۲ test for categorical variables. The genotype frequencies were ۷.۶% for GG, ۸۰.۳% for AG and ۹.۱% for AA in control group, and ۱۵% for GG, ۵۳% for AG and ۳۲% for AA among the patients.. Based on these data, there was significant differences in the genotypes and allele frequencies between cases and control. Our results suggest circfoxo۳ rs۱۲۱۹۶۹۹۶ polymorphism is associated with CRC and the GG genotype may be a risk factor for the disease. Further studies are needed to confirm the results