Colorimetric Chemsensor Based on ArsenazoIII for Determination and Naked-Eye Detection of Argenin in Aqueous Solutions

Arginine (Arg) is a semi-essential amino acid in humans that plays an important role in maintaining balance hormones, strengthening the immune system, wound healing and tissue integrity. Excessive amounts can cause allergies, loss of consciousness, low blood pressure and swelling of the limbs Therefore, determining Arg and knowing the proper intake of this amino acid is essential for maintaining human health. In this paper, spectrophotometric and colorimetric method for the determination of Arg in aqueous medium using ArsenazoIII (AAIII) is described.Adding Arg to AAIII as chemosensor causes a visible color change from red to purple and decrease of absorbance at ۵۳۶ nm which related interaction between of AAIII and Arg. The linear dynamic range was achieved for UV-Vis absorbance experiment were ۱۵-۳۴۰ μmolL-۱. The limits of detection was also calculated to ۰.۰۴ μmolL-۱. This method was employed for the determination of Arg in environmental samples which gave satisfactory results.