The effects of Xylose monosaccharide on Water Buffalo (Bubalus bubalis) epididymal sperm kinetic parameters at ۳۷ ˚ C

The objective of this study was to test xylose monosaccharide sugar on buffalo epididymal sperm kinetic parameters at ۳۷ ˚ C, of up to ۲۴ h. Testes with epididymis were collected after death at urmia industrial abattoir, testes were recovered within ۳۰ min after slaughter. Paired cauda epididymides from each mature buffalo bull were sliced and sperms transferred into tubes containing tissue culture medium (TCM, ۱۹۹) with ۱۰% bovine serum albumin at ۳۷ ˚ C. Different levels of xylose (۰,۱, ۳, ۵, ۱۰, ۱۵ mM) were added into TCM-۱۹۹ containing sperms (۴۰-۵۰×۱۰۶ sperm/ml), with ۱۰%, bovine serum albumin. Then samples were incubated at ۳۷ ˚ C. Thereafter, at ۱,۳,۵,۷,۱۰,۱۲ and ۲۴ hours incubation times, kinetics of at least ۱ thousand sperms per samples were recorded with computer assisted sperm analysis (CASA). Results revealed that the motility parameters till ۱۲ hrs did not show any significant differences between groups, actually no significant difference was observed between control and xylose groups means .But at ۲۴ h, after incubation the means of xylose levels were lower than that of the control specially at ۱۵ mM of xylose level the difference with control was significant (p<۰.۰۵). It is concluded that the xylose at high levels significantly reduce the kinetic parameters of buffalo bull epididymal sperm, especially at ۲۴ h after incubation. Therefore, the usage of xylose sugar and at the high levels is not recommended for the energy supply of Buffalo bull epididymal sperm at ۳۷ ˚ C incubation in TCM ۱۹۹.