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مقالات فارسیISIکنفرانسهاژورنالها

Xeno-free generation of new Yazd human embryonic stem cell lines (Yazd۴-۷) as a prior stage toward good manufacturing practice of clinical-grade raw materials from discarded embryos: A lab resources report

محل انتشار: مجله طب تولید مثل ایران، دوره: 21، شماره: 8
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 67

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لینک ثابت به این مقاله:
https://civilica.com/doc/1779643

شناسه ملی سند علمی:

JR_IJRM-21-8_002

تاریخ نمایه سازی: 22 مهر 1402

چکیده مقاله:

Background: Xeno-free generation of human embryonic stem cells (hESCs) is important to prevent potential animal contaminations in culture for advanced cell-based therapeutic applications. Xeno-free production of hESCs is the first step for manufacturing clinical-grade hESC lines. Objective: To produce new hESC lines in xeno-free condition. Materials and Methods: This lab resources report was conducted at Stem Cell Biology Research Center, Yazd, Iran from ۲۰۱۹-۲۰۲۲. ۴ new hESC lines from ۱۱ (۱۰ fresh and ۱ frozen) donated surplus discarded human embryos were established. In this study, we report the xeno-free derivation of new Yazd hESC lines (Yazd۴-۷), without using immunosurgery, by culturing intact zona-free blastocysts obtained from discarded embryos onto the YhFF#۸ cells as a feeder layer in a microdrop culture system. The pluripotency gene expression profile of the cell lines was assessed by reverse transcription polymerase chain reaction and the expression of specific surface markers was detected using immunofluorescent staining. In vitro differentiation was induced using embryoid body formation and gene expression profile of ۳ germ layers and germ cells. Reverse transcriptase polymerase chain reaction was investigated to prove their pluripotent capacity. Results: In sum, we have been able to generate ۴ new hESC lines (Yazd۴-۷) from ۱۱ discarded embryos in xeno-free culture conditions using a micro drop culture system and YhFF#۸ as a human source feeder layer. Conclusion: The outcome of this work can be the foundation for the future allogeneic cell-based therapeutic application using clinical grade good manufacturing practice-derived hESC derivatives.

کلیدواژه ها:

Derivation ، Human embryonic stem cells ، Human foreskin fibroblasts ، Xeno-free ، Good manufacturing practice ، Mouse embryonic fibroblasts. ، تولید ، سلول های بنیادی رویانی انسانی ، فیبروبلاست های پوست ختنه انسانی ، Xeno-free ، تولید خوب ، سلول های فیبروبلاست جنینی موشی.

نویسندگان

Fatemeh Hajizadeh-Tafti

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Jalal Golzadeh

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Fatemeh Akyash

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Resear

Somayyeh-Sadat Tahajjodi

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Resear

Ehsan Farashahi-Yazd

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Hassan Heidarian-Meimandi

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Behrouz Aflatoonian

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Resear

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