Characterization of ۱۶SrII group phytoplasmas associated with alfalfa (Medicago sativa) witches’ broom disease in diverse areas of Iran
محل انتشار: Journal of Crop Protection، دوره: 5، شماره: 4
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51
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شناسه ملی سند علمی:
JR_JCP-5-4_009
تاریخ نمایه سازی: 13 آبان 1402
چکیده مقاله:
Alfalfa witches’ broom (AWB) is one of the most important alfalfa diseases in Iran. To characterize ۱۶SrII group phytoplasmas associated with this disease, symptomatic and asymptomatic plants were collected during ۲۰۱۳-۲۰۱۵ and subjected to direct and nested polymerase chain reaction (PCR) using P۱/P۷, R۱۶mF۲/R۱۶mR۲ and R۱۶F۲n/R۱۶R۲. PCR amplicons of ~۱.۸, ~۱.۴ and ~۱.۲۵ kb respectively, were obtained only from all symptomatic plants. Restriction fragment length polymorphism (RFLP) analysis of R۱۶F۲n/R۲ amplicons showed that the phytoplasma associated with AWB disease were members of ۱۶SrII group subgroups ۱۶SrII-D and -C. Blast analysis of these amplicon sequences and sequence homology of collected strains and strain sequences retrived from GenBank (AWB strains Chahgeer, Juyom and Bushehr) confirmed that AWB phytoplasmas collected from Bafg, Ardakan, Bahabad and Herat (Yazd province), Nikshahr (Sistan-Baluchestan), Bam, Zarand, Jiroft (Kerman province), Bushehr (Bushehr province), Tabas (South Khorasan province), Jowkar (Hamedan province) and Zardenjan (Esfahan province) cluster with phytoplasma strains enclosed in the ۱۶SrII-D subgroup, while AWB strains from Chahgeer (Yazd province) and Juyom (Fars province) cluster with phytoplasma strains in the ۱۶SrII-C subgroup. Based on these results the predominant strains of ۱۶SrII phytoplasmas associated with AWB disease in Iran were classified in the ۱۶SrII-D subgroup. In Ashkezar and Abarkouh in Yazd province entire alfalfa farm was infected with witches’ broom disease. In ۳ year alfalfa stands in Ashkezar alfalfa farms were plowed due to high incidence of the disease.
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نویسندگان
Seyyed Alireza Esmailzadeh Hosseini
Plant Protection Department, Bu-Ali Sina University, Hamedan, Iran, Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran.
Gholam Khodakaramian
Plant Protection Department, Bu-Ali Sina University, Hamedan, Iran.
Mohammad Salehi
Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Zarghan, Iran.
Assunta Bertaccini
Department of Agricultural Sciences, Alma Mater Studiorum, University of Bologna, Italy.
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