Differences in CpG island distribution between exogenous and endogenous jaagsiekte sheep retrovirus strains

سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 67

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شناسه ملی سند علمی:

JR_VRFAN-14-10_002

تاریخ نمایه سازی: 15 آبان 1402

چکیده مقاله:

The jaagsiekte sheep retrovirus (JSRV), belonging to the betaretrovirus genus of the retroviridae family, includes both exogenous and endogenous jaagsiekte sheep retroviruses (exJSRV and enJSRV, respectively). At the proviral genome level, exJSRV and enJSRV strains have a high degree of similarity with their main variation regions being the LTR, gag, and env genes. In this study, for the first time, we investigated and compared the distribution of CpG islands between these enJSRV and exJSRV strains. Specifically, we analyzed a total of ۴۲ full-length JSRV genomic sequences obtained from the GenBank® database to identify CpG islands in the exJSRV and enJSRV genomes using the MethPrimer software. Our results showed that the CpG islands in the two JSRV strains were mainly distributed in the LTR, gag, and env genes. In exJSRVs, ۶۶.۶۶% (۶/۹), ۳۳.۳۳% (۳/۹), and ۱۰۰% (۹/۹) of the sequences presented at least one CpG island in LTR, gag, env genes, respectively, and for enJSRVs, ۸۴.۸۴% (۲۸/۳۳), ۵۷.۵۷% (۱۹/۳۳), and ۹۶.۹۶% (۳۲/۳۳) of the sequences presented at least one CpG island in the LTR, gag, and env genes. These findings suggested that the distribution, length, and genetic traits of CpG islands were different for the exJSRV and enJSRV strains. In future, it would be necessary to demonstrate the biological significance of CpG islands within these genes in exJSRV and enJSRV genomes. This will enhance understanding regarding the potential role of CpG islands in epigenetic regulation.

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نویسندگان

Qiang Liu

Department of Agricultural Science and Technology, Nanchong Vocational and Technical College, Nanchong, China

Yue-Yang Yu

Department of Agricultural Science and Technology, Nanchong Vocational and Technical College, Nanchong, China

Huai-Yu Wang

Department of Agricultural Science and Technology, Nanchong Vocational and Technical College, Nanchong, China

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