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مقالات فارسیISIکنفرانسهاژورنالها

Enhancement of Lytic Enzymes Activity and Antagonistic Traits of Trichoderma harzianum Using γ-Radiation Induced Mutation

محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 21، شماره: 4
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51

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https://civilica.com/doc/1817168

شناسه ملی سند علمی:

JR_JASTMO-21-4_020

تاریخ نمایه سازی: 23 آبان 1402

چکیده مقاله:

Trichoderma species are known as effective agents used for biological control of plant pathogenic fungi. The Trichoderma harzianum and its mutant isolates were cultured and their traits including, mycelial growth, antagonistic activity and extracellular proteins and enzymes production (Chitinase and Cellulase) were investigated to select the most effective mutant isolates against plant pathogenic fungus Rhizoctonia solani. Also, the purity and composition of enzyme-rich protein samples were evaluated under denaturing gel electrophoresis. This study clearly showed the possibility of improving mycelia growth rate (from ۱.۱۸ to ۱.۳۳ cm d-۱), the antagonistic capability of Trichoderma (from ۵۴.۹% growth inhibition of R. solani to ۶۶%), extracellular proteins and enzymes production for biological control of plant diseases through mutation with γ-radiation. Also, compared to wild type strain, protein production in the mutant isolates increased. Moreover, the highest specific chitinase enzyme activities were observed in mutant isolates T. h M۸ (۴۲.۴۸ U mg-۱) and T. h M۱۵ (۳۸.۲۵ U mg-۱). Trichoderma mutant of T. h M۸ maintained higher mycelia growth rate and higher ability to inhibit growth of R. solani. The SDS-PAGE profiles had several enzyme protein bands such as CelloBioHydrolases (CBHs), EndoGlucanases (EGs), β-Glucosidases (BGLs), endochitinases, and β-(۱, ۴)-N-acetyl glucoaminidases. SDS-PAGE analysis indicated the presence of different protein bands in the range of ۱۰.۵ to ۲۴۵ KDa. Interestingly, expression of chitinase in ۹۵ percent of mutants was higher than wild type of T. harzianum. The results showed that gamma mutation could increase the efficiency and amount of enzymes in T. harzianum, while these enzymes are involved in antagonistic properties of T. harzianum.

کلیدواژه ها:

Cellulase ، Chitinase ، Gamma radiation ، Mutation.

نویسندگان

S. Ghasemi

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

N. Safaie

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

S. Shahbazi

Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of IRAN (AEOI), Alborz, Islamic Republic of Iran.

M. Shams-Bakhsh

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

H. Askari

Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of IRAN (AEOI), Alborz, Islamic Republic of Iran.

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