Isolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 7، شماره: 4
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 29
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شناسه ملی سند علمی:
JR_VRFAN-7-4_011
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Ornithobacterium rhinotracheale (ORT) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. The objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ORT bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in Khuzestan province, south-west of Iran. A total of ۲۱۰ tracheal swab samples were collected from ۲۱ broiler flocks slaughtered in abattoirs of the province. The results of cultural and biochemical tests showed that ۲۳ (۱۰.۹۵%) isolates from tracheal swabs of ۴ flocks (۱۹.۰۴%) were identified as ORT, but according to molecular characterization, ۱۸ (۸.۵۷%) ORT isolates were positive in PCR assay and produced the predicted ۷۸۴ bp amplification product. Finally, using the disk diffusion method, the drug resistance patterns of ORT isolates were determined against a panel of commonly used antimicrobial agents. Antimicrobial susceptibility test revealed that all isolates (۱۰۰%) were sensitive to tetracycline, florfenicol and cephalexin. The highest antimicrobial resistance (۸۹.۰۰%) was seen for fosfomycin, sultrim and gentamicin. The results of present research showed that there was significant difference between the isolation rates of ORT from various areas of the province. As well, our findings indicated that the simultaneous use of both cultural and molecular techniques results in more comprehensive outcomes in the isolation and identification of the organismfrom understudy hosts.
کلیدواژه ها:
نویسندگان
Mansour Mayahi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Darioush Gharibi
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Rahim Ghadimipour
PhD Candidate of Bacteriology, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Forough Talazadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
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