Prevalence and molecular characterization of staphylococci isolated from sheep with subclinical mastitis in West-Azerbaijan province, Iran
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 7، شماره: 2
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 49
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شناسه ملی سند علمی:
JR_VRFAN-7-2_010
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-۳-phosphate dehydrogenase (gap) gene. Also, antibiotic resistance of staphylococcal isolates against different antibiotics was investigated. A total number of ۹۰۰ milk samples from ۴۵۰ native ewes in their mid-lactation period were examined by the California mastitis test (CMT). The CMT positive samples were cultured and bacteria were isolated from ۸۶ (۹.۵۰%) glands and ۷۴ (۱۶.۴۰%) ewes. The prevalence of subclinical mastitis in the examined ewes was ۱۶.۴۰%. Microbiological analysis of milk samples revealed that ۲۷ out of ۷۴ sheep with subclinical mastitis were infected with Staphylococcus spp. Amplification of gap gene of ۲۷ Staphylococcus isolates generated a single amplicon of ۹۳۳ bp in size confirming that isolates were belonged to Staphylococcus genus. Digestion of PCR products by AluI endonuclease generated different RFLP patterns for each species. Nucleotide sequencing of gap gene followed by phylogenetic analysis showed that the most dominant Staphylococcusspecies were S. epidermidis, S. xylosus and S. chromogenes. Staphylococcal isolates showed the highest resistance to penicillin and ampicillin. In conclusion, Staphylococcus species, except for the southern parts of the province, play an important role in the development of subclinical mastitis in sheep in West-Azerbaijan province of Iran. Also, chloramphenicol, ciprofloxacin and neomycin are the most effective antibiotics for treatment of this disease.
کلیدواژه ها:
نویسندگان
Bentolhoda Rahman
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abdolghaffar Ownagh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Karim Mardani
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Farhad Farrokhi Ardebili
Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
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