Comparing protective effect of grape seed extract versus atorvastatin on endometriosis in rat model: Evidence for immunohistochemical and biochemical alterations
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 6، شماره: 2
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 47
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شناسه ملی سند علمی:
JR_VRFAN-6-2_002
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Thirty six Wistar albino rats with implant induced endometriosis were randomly divided into six groups of six animals each. The rats in the first group received nothing and were euthanized at day ۲۱. In the second group, rats received nothing and were euthanized at day ۳۶. The third group received atorvastatin (ATV; ۵ mg kg-۱ per day, orally) until ۲۱ days from induction of endometriosis, and the fourth group received ATV from the ۱۵th day after induction of endometriosis for ۲۱ days. The fifth group received grape seed extract (GET; ۴۵۰ mg kg-۱ per day, orally) until ۲۱ days from induction of endometriosis. In the sixth group, GET was administered from the ۱۵th day after induction of endometriosis for ۲۱ days. The estrogen receptor positive cells (ER+) distribution and angiogenesis were assessed using immunohistochemical and immunoflourescent analyzes, respectively. The active cells with intracytoplasmic carbohydrate content were analyzed. Erα mRNA expression was assessed using semiquantitative real time-PCR and the tissue levels of malondialdehyde (MDA), glutathione peroxidase (GSH-px) and superoxide dismutase (SOD) were evaluated. The GET and ATV-treated animals showed significant reduction in endometriosis-increased ER+ cells distribution as well as significant decrease in Erα mRNA levels (p < ۰.۰۵). Our data suggests that GET exerts a potent inhibitory effect on development of endometriotic implants similar to ATV.
کلیدواژه ها:
نویسندگان
Arash Kakaiy
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Esmail Ayen
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rajabali Sadrkhanlou
Department of Basic Sciences Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Farshid Sarrafzadeh-Rezaei
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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