Evaluation of antioxidant effects of crocin on sperm quality in cyclophosphamide treated adult mice
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 5، شماره: 3
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 44
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شناسه ملی سند علمی:
JR_VRFAN-5-3_009
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Cyclophosphamide (CP) is one of the anti-neoplastic drugs. Despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. This study was aimed to investigate the protective effect of crocin in improving the toxicity induced by CP in reproductive system. In this study, ۲۴ male adult mice (۶ to ۸ weeks) were randomly divided into three groups, control group received normal saline (۰.۱ mL, IP, daily), the CP group received CP (۱۵ mg kg-۱, IP, weekly) and the CP + crocin group received CP along with crocin (۲۰۰ mg kg-۱, IP, daily). After ۳۵ days of treatment, animals were sacrificed. The samples of epididymis in human tubal fluid medium incubated for ۳۰ min in ۵% CO۲ for flotation of sperm. Sperm were obtained from caudal epididymis using dissecting method. Then, the parameters of sperm quality including sperm count, motility, viability, DNA damage, nuclear maturation, and sperm morphology were evaluated. In CP group, the sperm count, motility, viability, nuclear maturation and sperm morphology were significantly decreased compared to control group (p < ۰.۰۵) and in the CP + crocin group all of these parameters significantly increased compared to CP group (p < ۰.۰۵). The percentage of sperm with DNA damage in the CP group significantly increased compared to other groups (p < ۰.۰۵). The results of this study indicated that the crocin was able to suppress free radicals and enhance the quality of sperm in CP treated animals.
کلیدواژه ها:
نویسندگان
Zahra Bakhtiary
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rasoul Shahrooz
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abbas Ahmadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Leila Zarei
Maternal and Childhood Obesity Research Center, Urmia University of Medical Sciences, Urmia, Iran
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