A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51

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شناسه ملی سند علمی:

JR_VRFAN-8-4_005

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

A total number of ۴۵۰ blood samples were collected from ۴۵ different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in ۴.۲%, while ۸.۹% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in ۳۷/۴۰ (۹۲.۵%) and ۳/۴۰ (۷.۵%) samples, respectively. ۵۳۰ ticks of ۱۰ Ixodid species were collected from the same cattle. Hyalomma anatolicum was the most prevalent tick species (۱۹.۹%). An expected ۵۲۰ bp fragment of Babesia spp. was generated in ۲۲ (۴۸.۸%) of Rhpicephalus annulatus, ۱۸ (۴۰.۰%) of R. bursa and ۱۲ (۳۰.۰%) R. sanguineus sensu lato. The mPCR findings revealed that all infected ticks including R. annulatus, R. bursa and R. sanguineus were totally infected with B. bigemina. The DNA amplification of B. bovis and B. bigemina in egg samples showed that only B. bigemina was detected in two specimens of R. annulatus. It could be concluded that B. bigemina was the dominant causative agent in this region but the evidence of B. bovis infection of cattle in a few cases was noted, as well. The results suggested that B. bigemina and B. bovis could be detected in the DNA extracted from R. annulatus, R. bursa and R. sanguineus sensu lato confirming previous reports. Since B. bigemina is transmitted transovarially by R. annulatus, it might act as an important vector for B. bigemina.

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نویسندگان

Sepideh Rajabi

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Bijan Esmaeilnejad

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Mousa Tavassoli

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

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  • Terkawi MA, Alhasan H, Huyen NX, et al. Molecular and ...
  • Bilgiç HB, Karagenç T, Simuunza M, et al. Development of ...
  • Liu J, Guan G, Liu A, et al. A PCR ...
  • Esmaeilnejad B, Tavassoli M, Asri-Rezaei S, et al. Determination of ...
  • Estrada-Pena A, Bouattaur A, Camicas JL, et al. Ticks of ...
  • Oliveira-Sequeira TCG, Oliveira MCS, Araujo JP, et al. PCR-based detection ...
  • Georges K, Loria GR, Riili S, et al. Detection of ...
  • Fakhar M, Hajihasani A, Maroufi S, et al. An epidemiological ...
  • Noaman V. A molecular study on Theileria and Babesia in ...
  • Ekici, OD, Sevinc F. Comparison of cELISA and IFA tests ...
  • Ziapour SP, Esfandiari B, Youssefi MR. Study of the prevalence ...
  • Khamesipour F, Doosti A, Koohi A, et al. Determination of ...
  • Ibrahim O, Taha Z, Jassim S. Prevalence of Babesia bovis ...
  • Altay K, Aydin MF, Dumanli N, et al. Molecular detection ...
  • Acici M, Bölükbaş CS, Pekmezci GZ, et al. Sero-epidemiological survey ...
  • Aktas M, Ozubek S. Molecular and parasitological survey of bovine ...
  • Saleh MA. Erythrocytic oxidative damage in crossbred cattle naturally infected ...
  • Yavuz A, İnci A, Düzlü Ö, et al. Molecular characterization of Babesia bovis ...
  • Rodriguez AE, Florin-Christensen M, Flores DA, et al. The glycosylphosphatidylinositol-anchored ...
  • Ozlem DE, Sevinc F. Seroepidemiology study of B. bigemina in ...
  • Sevgili M, Cakmak A, Gokcen A, et al. Prevalence of ...
  • Ameen KAH, Abdullah BA, Abdul-Razaq RA. Sero-prevalence of Babesia bigemina ...
  • Calder JA, Reddy GR, Chieves L, et al. Monitoring Babesia ...
  • Bell-Sakyi L, Koney EBM, Dogbey O, et al. Incidence and ...
  • Oliveira MC, Oliveira-Sequeira TC, Araujo JP Jr, et al. Babesia spp. infection in ...
  • Terkawi MA, Huyen NX, Shinuo C, et al. Molecular and ...
  • Zintl A, Gray JS, Skerrett HE, et al. Possible mechanisms ...
  • Sappenfield E, Jamieson DJ, Kourtis AP. Pregnancy and susceptibility to ...
  • Theodoropoulos G, Gazouli M, Ikonomopoulos JA, et al. Determination of ...
  • Ibrahim HM, Adjou Moumouni PF, Mohammed-Geba K, et al. Molecular ...
  • Laha R, Mondal B, Biswas SK, et al. Detection of ...
  • Ica A, Inci A, Yildirim A. Parasitological and molecular prevalence ...
  • Inci A, YazarS, Tuncbilek AS, et al. Vectors and vector-borne ...
  • Yıldırım A, Düzlü Ö, Inci A, et al. Comparative diagnosis ...
  • Nabian S, Rahbari S. Occurrence of soft and hard ticks ...
  • Tavassoli M, Tabatabaei M, Mohammadi M, et al. PCR-based detection ...
  • Sohrabi S, Yakhchali M, Ghashghai O. Hard ticks (Acarina: Ixodidae) ...
  • Abdigoudarzi M. Detection of naturally infected vector ticks (Acari: Ixodidae) ...
  • Omer LT, Kadir MAA, Seitzer U, et al. A survey ...
  • Dumanli N, Aktas M, Cetinkaya B, et al. Prevalence and ...
  • Aktas M, Altay K, Ozubek S, et al. A Survey ...
  • Riabi H, Atarodi A. Faunistic study of hard ticks (Ixodidae) ...
  • Gharekhani J, Gerami-Sadeghian A, Sadeghi-Dehkordi Z, et al. Determination of ...
  • Aydin MF, Aktas M, Dumanli N. Molecular identification of Theileria ...
  • Hunfeld KP, Hildebrandt A, Gray JS. Babesiosis: recent insights into ...
  • Yeruham I, Hadani A, Galker F. Some epizootiological and clinical ...
  • Yakhchali M, Rostami A, Esmaelzadeh M. Diversity and seasonal distribution ...
  • Bakirci S, Sarali H, Aydin L, et al. Distribution and ...
  • Howell JM, Ueti MW, Palmer GH, et al. Transovarial transmission ...
  • Chauvin A, Moreau E, Bonnet S, et al. Babesia and ...
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