Effect of Satureja khuzestanica essential oil against fertility disorders induced by busulfan in female mice

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 48

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شناسه ملی سند علمی:

JR_VRFAN-8-4_002

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. Satureja khuzestanica has antioxidant effects. The aim of this study was to examine whether S. khuzestanica essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four adult female mice were divided into six groups including dimethyl sulfoxide (control), SKEO ۲۲۵.۰۰ mg kg-۱ (orally), busulfan ۳.۰۰ mg kg-۱ (orally), busulfan ۳۶.۰۰ mg kg-۱ (intraperitoneally), busulfan ۳.۰۰ mg kg-۱ and SKEO and busulfan ۳۶.۰۰ mg kg-۱ and SKEO. After ۲۸ days, the mice were euthanized and oocytes were removed for in vitro fertilization (IVF) rate evaluation. Oocyte quantity and quality, fertilization rate and pre-implantation embryo development were daily examined with a stereo microscope in a period of ۱۲۰ hr. Serum levels of estradiol and progesterone were also evaluated. Busulfan caused significant decreases in oocyte number and quality, fertilization rate, pre-implantation embryo development and embryo quality. The SKEO significantly decreased the adverse effects of busulfan. The present study indicated that SKEO can protect female fertility potential against busulfan induced damages.

نویسندگان

Abbas Ahmadi

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Saleh Bamohabat Chafjiri

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rajab Ali Sadrkhanlou

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

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