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Molecular characterization and phylogenetic analysis of feline hemoplasmas in domestic cats in Iran

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 8، شماره: 1
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 54

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https://civilica.com/doc/1818256

شناسه ملی سند علمی:

JR_VRFAN-8-1_011

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Three known feline hemoplasmas are Mycoplsama haemofelis, ‘Candidatus Mycoplasma haemominutum’ and ‘Candidatus Mycoplasma turicensis’. They are described as cause of feline infectious anemia in domestic and wild felids. Other blood parasites or blood-related pathogens like concurrent retroviral infections may deteriorate the clinical condition and severity of anemia. The aims of this study were molecular characterization and phylogenetic analysis of hemoplasmas in domestic cats in Iran for the first time. Blood samples were collected from ۱۸۵ healthy and diseased domestic cats. Blood smears were prepared and hematological parameters were measured to determine possible anemia. Using ۱۶S rRNA gene universal and species specific polymerase chain reactions with the following sequencing, ۴۷ (۲۵.۴۰%) of cats were hemoplasma positive. Also, ۱۷.۰۲%, ۷۲.۵۰% and ۴۰.۴۰% of total positive samples were M. haemofelis, ‘Ca. M. haemominutum’ and ‘Ca.M. turicensis’ infected, respectively. ۱۰ (۲۱.۲۰%) of hemoplasma positive cats had anemic blood profiles (HCT < ۲۴.۰۰%). All M. haemofelis infected cases were included. Partial ۱۶S rRNA gene phylogenetic analysis revealed a high identity between the hemoplasma species found in this study and domestic cat sequences existing in GenBank. Phylogenetic analysis revealed ۹۴.۰۰% to ۱۰۰% sequence identity between sequences of this study and existing sequences in Genbank. All hemoplasma isolates in this study were grouped within a single clade and additionally subdivided into two groups; haemofelis group including M. haemofelis and ‘Ca. M. turicensis’ and haemominutum group including ‘Ca. M. haemominutum’.

کلیدواژه ها:

Anemia ، Cat ، Feline hemoplasma ، Iran

نویسندگان

Fereshteh Ghazisaeedi

Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Nahid Atyabi

Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Taghi Zahraei Salehi

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Iraj Ashrafi Tamai

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Saeid Tabatabaei

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Solmaz Chegeni

Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

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