Histopathology and molecular identification of Sarcocystis species forming macrocysts in slaughtered sheep and goats of Duhok, Iraq
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 8
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 58
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-14-8_002
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
This study aimed to identify Sarcocystis species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of ۲۶۰ macrosarcocyst samples were randomly collected from ۱,۳۳۷ infected sheep and goats slaughtered at different abattoirs in Duhok province, Iraq, from May ۲۰۲۱ to June ۲۰۲۲. The macroscopic cysts, which were found in the esophagus, diaphragm, and abdominal muscles, were classified into fat and thin cysts. Histopathological examination of the observed fat and thin cysts showed a thick eosinophilic wall, several internal septa-forming compartments enclosing numerous bradyzoites, and mild mononuclear inflammatory cells infiltrating around the cysts. The ۱۸ Subunit ribosomal RNA (۱۸S rRNA) and ۲۸ Subunit ribosomal RNA (۲۸S rRNA) genes of Sarcocystis spp. were amplified by polymerase chain reaction (PCR) from ۲۰۰ macrosarcocysts samples. Molecularly, the DNA sequencing results obtained from fat macrocysts of sheep and goats were found to be identical to Sarcocystis gigantea, and from thin cysts of sheep proved to be similar to Sarcocystis medusiformis, while from thin macrocysts of goats were found to be identical to Sarcocystis moulei. Alignment and phylogenetic analysis observed a very close relationship between identified species of Sarcocystis and other Sarcocystis DNA sequences of sheep and goats across the world. To our knowledge, this is the first histopathological and molecular study for identification of Sarcocystis spp. isolated from different macroscopic forms of sarcocysts of sheep and goats in Iraq.
کلیدواژه ها:
نویسندگان
Shivan Hussein
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Duhok University, Duhok, Iraq
Assel Ibrahim
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Duhok University, Duhok, Iraq
Mohammed Shukur
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Duhok University, Duhok, Iraq
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :