Physiological properties of indigenous lytic bacteriophages as monophage suspension and cocktail against poultry-adapted typhoidal Salmonella variants

Rida Durrani; Ali Sheikh; Masood Rabbani; Muti-ur-Rehman Khan; Muhammad Riaz; Muhammad Naeem; Salman Chattha; Aleena Kokab; Munazzah Maqbool; Muhammad Abbas; Naila Siddique

Physiological properties of indigenous lytic bacteriophages as monophage suspension and cocktail against poultry-adapted typhoidal Salmonella variants

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 13، شماره: 4

سال انتشار: 1401

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 59

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https://civilica.com/doc/1818467

شناسه ملی سند علمی:

JR_VRFAN-13-4_004

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

The emergence and spread of multidrug resistance among pathogens of the agro-food sector is increasing at an alarming rate, which has directed attention to the search for alternative to antibiotic therapy. The present work studied the physiological and population dynamics of lytic bacteriophages against avian-adapted Salmonella. Out of ۲۸ positive samples, four bacteriophage isolates (SalØ-ABF۳۷, SalØ-RCMPF۱۲, SalØ-MCOH۲۶, SalØ-DNLS۴۲) were selected based on their ability to clearly lyse bacterial test strains. The isolates propagated were active against closely related D۱ serotypes, i.e., S. Enteritidis and S. Typhimurium, with no heterologous activity against Escherichia coli ATCC ۲۵۹۲۲ and Staphylococcus aureus ATCC ۲۳۲۳۵. Each of the monophage suspension and cocktail efficiently suppressed the bacterial count from exponential increase in comparison to the untreated bacterial control. The bacterial turbidity was recorded as ۰.۲۴۴ at λ۶۰۰ during ۴۰۰ min of co-incubation, in contrast to bacterial control showing λ۶۰۰ = ۰.۶۶۹. The latent period was recorded to be ۲۵, ۳۵, ۲۵ and ۳۰ for SalØ-ABF۳۷, SalØ-RCMPF۱۲, SalØ-MCOH۲۶ and SalØ-DNLS۴۲, with ۷۳.۰۰, ۹۷.۰۰, ۱۳۲ and ۷۵.۰۰ PFU cell-۱, respectively. The highest lytic activity was seen at ۳۷.۰۰ ˚C - ۴۲.۰۰ ˚C, with phage particle count being fairly stable at pH ۳.۰۰ - ۹.۰۰. Each of the isolates possessed dsDNA by being resistant to RNase A. The current study concludes that lytic phages are promising alternative to combat multidrug resistant superbugs. The physiological characterization and bacterial growth inhibition are important parameters in standardization of phage therapy.

کلیدواژه ها:

Bacteriophages ، Multidrug Resistant ، Phage therapy ، Salmonella

نویسندگان

Rida Durrani