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مقالات فارسیISIکنفرانسهاژورنالها

Molecular detection and analysis of virulence genes in multi-drug resistant Escherichia coli from infected broilers

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 4
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 56

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1818474

شناسه ملی سند علمی:

JR_VRFAN-12-4_017

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Escherichia coli associated infections are major threats in poultry industry owing to severe economic losses each year. This study was conducted to identify E. coli isolates, to evaluate their antibiotic sensitivity and to find out their virulence patterns from infected broilers of Sylhet city in Bangladesh. Using polymerase chain reaction, a total ۲۰ isolates were identified as E. coli from ۱۱ chickens, exhibiting symptoms like colibacillosis and/or diarrhea. All isolates were positive for type-۱ fimbrial adhesion (fimH), followed by putative avian hemolysin (hlyF) in ۱۷ isolates; while none of the isolates was amplified with intimin (eaeA). Among ۱۰ tested antibiotics, ۱۰۰% of the isolates (n = ۲۰) showed resistance to ampicillin, amoxicillin and tetra-cycline; but they were ۱۰۰% sensitive to gentamicin. Organ specific correlations of antibiotic sensitivity were obtained among the isolates through principal component analysis (PCA) and Agglomerative Hierarchical Clustering (AHC). The ۱۶S rRNA data of two multi-drug resistant isolates revealed closed clustering with clinical E. coli strains which could be indication of their zoonotic potential. In conclusion, the results depict higher prevalence of fimH and hlyF genes and drug resistance patterns of E. coli isolates from broilers in Sylhet city of Bangladesh.

کلیدواژه ها:

۱۶S rRNA sequencing ، Commercial broilers ، Molecular detection ، Multi-drug resistant E. coli ، Virulence genes

نویسندگان

Kazi Azim

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Saneya Somana

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Md Hasan

Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

Md Foysal

Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

Md Ali

Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

Tanjia Chowdhury

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Md Nazmul Hossain

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

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