ورود
مقالات فارسیISIکنفرانسهاژورنالها

DNA damage in dental pulp mesenchymal stem cells: An in vitro study

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 9، شماره: 4
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 47

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1818504

شناسه ملی سند علمی:

JR_VRFAN-9-4_001

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for clinical application of cellular therapy for this type of cell. The cell culture was maintained with Dulbecco’s modified Eagle’s medium supplemented with ۱۰.۰۰% fetal bovine serum for eight passages. During each passage, cell proliferation, oxidative stress and level of DNA fragmentation were assessed by۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵-diphenyltetrazolium (MTT) assay, testing ۲,۷ dichlorodihydro-fluorescein-diacetate and PicoGreen®, respectively. There were important differences among the first three DPSC passages compared to passages ۴–۸ and a large number of nuclei with some levels of DNA damage (۳۰.۰۰ to ۴۰.۰۰% in initial DPSC passages and > ۵۰.۰۰% in late passages), indicating in vitro DPSC genomic fragility. Within the limitations of this study, the results suggest these relatively simple and inexpensive approaches - comet and DNA fragmentation assays - could help sort stem cells with less DNA damage for use in research or therapies.

کلیدواژه ها:

Dental pulp ، DNA damage ، Genotoxicity ، Mesenchymal stem cell ، Oxidative stress

نویسندگان

Jaime Sardá Aramburú Junior

Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Tiago Treichel

Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Saulo Tadeu Lemos Pinto Filho

Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

Sergio Gerhke

Biotecnos Research Center, Santa Maria, Rio Grande do Sul, Brazil; Catholic University of Uruguay, Montevideo, Uruguay

Alencar Kolinski Machado

Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Francine Carla Cadoná

Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Ivana Mânica da Cruz

Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil

Ney Pippi

Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Gronthos S, Mankani M, Brahim J, et al. Postnatal human ...
  • Seo BM, Miura M, Gronthos S, et al. Investigation of ...
  • Nakamura S, Yamada Y, Katagiri W, et al. Stem cell ...
  • Park JY, Jeon SH, Choung PH. Efficacy of periodontal stem ...
  • Kawashima N. Characterisation of dental pulp stem cells: A new ...
  • Cavazzana-Calvo M, Fischer A, Bushman FD, et al. Is normal ...
  • Baum C, Modlich U, Göhring G, et al. Concise review: ...
  • Roschke AV, Stover K, Tonon G, et al. Stable karyotypes ...
  • Draper BW, McCallum CM, Stout JL, et al. A high-throughput ...
  • Buzzard JJ, Gough NM, Crook JM, et al. Karyotype of ...
  • Hoffman LM, Carpenter MK. Human embryonic stem cell stability. Stem ...
  • Rebuzzini P, Neri T, Zuccotti M, et al. Chromosome number ...
  • Lefort N, Perrier AL, Laâbi Y, et al. Human embryonic ...
  • Lund RJ, Närvä E, Lahesmaa R. Genetic and epigenetic stability ...
  • Yang M, Büsche G, Ganser A, et al. Cytological characterization ...
  • Duailibi MT, Kulikowski LD, Duailibi SE, et al. Cytogenetic instability ...
  • Ribitsch I, Burk J, Delling U, et al. Basic science ...
  • Bernardi L, Luisi SB, Fernandes R, et al. The isolation ...
  • Rao MS, Mattson MP. Stem cells and aging: Expanding the ...
  • Wallace DR, Dodson SL, Nath A, et al. Delta opioid ...
  • Batel R, Jaksić Z, Bihari N, et al. A microplate ...
  • Georgiou CD, Papapostolou I, Grintzalis K. Protocol for the quantitative ...
  • Singh N, McCoy M, Tice R, et al. A simple ...
  • Tice RR, Agurell D, Anderson D, et al. Single cell ...
  • Hartmann A, Agurell E, Beevers C, et al. Recommendations for ...
  • Nadin S, Vargas-Roig L, Ciocca D. A silver staining method ...
  • Rocha CR, Lerner LK, Okamoto OK, et al. The role ...
  • Fung H, Weinstock DM. Repair at single targeted DNA double-strand ...
  • Impens S, Chen Y, Mullens S, et al. Controlled cell-seeding ...
  • Chen Y, Sonnaert M, Roberts SJ, et al. Validation of ...
  • Nikitina VA, Chausheva AI, Zhanataev AK, et al. Assessment of ...
  • Nagaria P, Robert C, Rassool FV. DNA double-strand break response ...
    • نمایش کامل مراجع