Efficacy of inactivated velogenic Newcastle disease virus genotype VII vaccine in broiler chickens
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 11، شماره: 2
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51
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شناسه ملی سند علمی:
JR_VRFAN-11-2_003
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Newcastle disease (ND) causes severe economic losses in poultry production. Despite the intensive vaccination regimes of NDV in Egypt, many outbreaks are being reported. The present study focused on the preparation and evaluation of inactivated velogenic Newcastle disease virus vaccine (genotype VII) isolated from Egyptian broiler chicken during ۲۰۱۵-۲۰۱۶. Fifty-five tissue samples including trachea, lung, liver, proventriculus, intestine, and kidney collected from commercial broiler chickens were used for virus isolation in specific pathogen-free embryonated chicken eggs (ECE) and identified using RT-PCR and sequencing. The isolates were classified by sequencing as velogenic NDV genotype VIId containing F۰ protein cleavage site motifs (۱۱۲RRQKRF۱۱۷). A selected isolate was served as a master seed for the preparation of inactivated NDV vaccine with or without Montanide ISA۷۰ adjuvant and evaluated in SPF chicks. Nine NDV isolates were isolated on ECE and the highest infectivity titer of the virus was ۷.۵۰ log۱۰ EID۵۰ mL-۱ by the ۵th passage. Vaccinated chicks with NDV-Montanide ISA۷۰ adjuvanted vaccine exhibited antibody titer of ۵.۲۰ log۲ at the ۳rd-week-post-vaccination (WPV) with the highest titer (۸.۹۰ log۲ mL-۱) at the ۶th-WPV. Protective antibodies values were persisted to ۱۲th WPV followed by a gradual decrease to the end of the experiment (۱۶th weeks). Vaccination of chicks with inactivated NDV isolate without adjuvant failed to induce protective HI antibodies all over the experiment. Chickens vaccinated with the ISA۷۰ adjuvant vaccine were passed homologous challenge tests with ۱۰۰% protective efficiency, while the unadjuvanted vaccine could not provide any protective efficiency. In conclusion, the preparation of inactivated oil adjuvant vaccine from NDV field circulating strains was efficient in controlling the disease in Egypt.
کلیدواژه ها:
نویسندگان
Mohamed Fawzy
Department of Virology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Rola Ali
Organization of Public Veterinary Services, Ismailia, Egypt
Wael Elfeil
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Amani Saleh
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt
Mokhtar Eltarabilli
Department of Virology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
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