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مقالات فارسیISIکنفرانسهاژورنالها

Goat paratuberculosis in Shiraz: Histopathological and molecular approaches

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 9، شماره: 3
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 59

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لینک ثابت به این مقاله:
https://civilica.com/doc/1818541

شناسه ملی سند علمی:

JR_VRFAN-9-3_008

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

In the present study, Mycobacterium avium subsp. paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from ۶۶ suspected goat carcasses to Johne’s disease were collected. Among ۶۶ examined slaughtered goats, nine (۱۳.۶۳%) goats were positive for MAP in both histopathological and PCR examinations. Eight goats were positive in PCR method while no lesion related to Johne’s disease was observed in their histopathological sections. All positive goats in histopathological examination were also positive in PCR. Based on the results of PCR, the detection rate of MAP in Shiraz abattoir was ۲۵.۸۰% (۱۷ goats). According to the present findings, although both histopathological and PCR methods are appropriate for detecting Johne’s disease, PCR is more sensitive than histopathological examination.

کلیدواژه ها:

Goat ، Histopathology ، Johne’s disease ، Mycobacterium avium subsp. paratuberculosis ، PCR

نویسندگان

Abdollah Derakhshandeh

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Fatemeh Namazi

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Elmira Khatamsaz

DVM Graduate, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Vida Eraghi

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Zahra Hemati

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

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