Effects of nano-selenium on mRNA expression of markers for spermatogonial stem cells in the testis of broiler breeder males
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 10، شماره: 2
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 67
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شناسه ملی سند علمی:
JR_VRFAN-10-2_007
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogonial stem cells (SSCs) in testis of broiler breeder males. A total of ۳۰ roosters of ۴۰ weeks age were randomly divided into ۵ groups. Groups were as follow: ۱) control (normal diet), ۲) diet supplemented with ۰.۳ mg/kg sodium Selenite, ۳) diet supplemented with ۰.۱۵ mg/kg Nano-Se, ۴) diet supplemented with ۰.۳ mg/kg Nano-Se and ۵) diet supplemented with ۰.۶ mg/kg Nano-Se. At the end of experimental period, birds autopsied and samples from testis of all birds were taken. The samples were used to examine the β۱-integrin (CD۲۹), thy-۱(CD۹۰ and NANOG mRNA expression by quantitative Real-Time PCR. The results of this study showed that testis of the groups fed with diets supplemented with ۰.۶mg/kg and ۰.۱۵mg/kg of Nano-Se had the highest and lowest mRNA expression of SSCs markers, respectively. In conclusion, the present study indicated that Nano-Se had advantages to sodium Selenite and ۰.۶ mg/kg of Nano-Se supplemented in males' diet in broiler breeders farms may contributes to optimal fertility via increasing mRNA expression of SSCs markers of roosters' testis and could be used to delay reduction of fertility caused by aging in broiler breeder males.
کلیدواژه ها:
نویسندگان
Seyed Sattar Jalali
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Alireza Talebi
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Manoochehr Allymer
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ali Soleimanzadeh
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mazdak Razi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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