Extra intestinal pathogenic Escherichia coli from human and avian origin: Detection of the most common virulence-encoding genes

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 38

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شناسه ملی سند علمی:

JR_VRFAN-10-1_007

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Pathogenic Escherichia coli strains cause a wide range of extra intestinal infections including urinary tract infection in humans and colibacillosis in poultry. They are classified into uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC) with genetic similarities and variations. Their pathogenicity is related to the virulence-encoding genes like sfa, papG II, ompT, iutA, and iss with zoonotic potentials. One hundred isolated E. coli from patients with urinary tract infection and ۱۰۰ E. coli from chickens with colibacillosis were evaluated for the presence of the most common virulence-encoding genes including sfa, papG II, ompT, iutA, and iss by multiplex polymerase chain reaction. While the frequency of sfa, papG II, ompT, iutA and iss encoding genes in APEC isolates were respectively ۰.۰۰%, ۶۷.۰۰%, ۶۳.۰۰%, ۸۹.۰۰% and ۸۹.۰۰%, the frequency of these encoding genes in UPEC isolates were ۱۸.۰۰%, ۴۰.۰۰%, ۴۰.۰۰%, ۷۴.۰۰% and ۴۸.۰۰%, respectively. Except for sfa, the frequencies of other encoding genes in APEC were more than those in UPEC isolates. The iutA as the most common UPEC encoding gene and iss as the most common APEC encoding gene were the most prevalent virulence factors in the examined E. coli isolates. Finding out the distribution of virulence-associated genes could be helpful to identify similarities and differences between APEC and UPEC isolates in order to provide more substantial evidence of their common virulence traits and potential zoonotic threats.

نویسندگان

Soheila Najafi

Student of Veterinary Medicine, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

Morad Rahimi

Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

Zahra Nikousefat

Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

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