Effects of new synthetic cryoprotectant agents on histological characteristics of various classes of vitrified bovine pre-antral follicles
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 10، شماره: 1
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 69
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شناسه ملی سند علمی:
JR_VRFAN-10-1_002
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovine ovarian tissue vitrification. From each bovine ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control group) or submitted to vitrification (sucrose, X-۱۰۰۰, Z-۱۰۰۰ and polyvinylpyrrolidone groups), either followed by in vitro culture for ۱ or ۵ days. In this case, although, the addition of X-۱۰۰۰ resulted in greater percentages of normal follicles for almost all pre-antral follicle classes compared to those of other groups, there are some exceptions. These results indicate that the inclusion of polyvinylpyrrolidone in the freezing media can improve the morphology of the post-warmed transitional follicles and cultured primordial follicles on day five more than other CPAs. According to the results of this study, it can be concluded that although ovarian tissue cryopreservation is often performed to preserve the primordial follicles, by choosing the best combination of permeating and non-permeating CPAs (synthetic polymers), more advanced stages of bovine pre-antral follicles, transitional, primary and secondary follicles, may also survive the cryopreservation process.
کلیدواژه ها:
نویسندگان
Mohammad Hamed Shahsavari
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Gholamali Moghaddam
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Hossein Daghigh Kia
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Ana Paula Ribeiro Rodrigues
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, Ceará, Brazil
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