Molecular detection and identification of Giardia duodenalis in cattle of Urmia, northwest of Iran

سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 55

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شناسه ملی سند علمی:

JR_VRFAN-9-1_012

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Giardia duodenalis is one of the most prevalent intestinal protozoa infecting humans and domestic animals. The aim of this study was to identify subspecies of G. duodenalis by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method from fecal samples of naturally infected cattle in the Urmia, West Azerbaijan province, Iran. Overall, ۲۴۶ fecal specimens were collected from the cattle (diarrheic and healthy) and microscopically examined for G. duodenalis. The PCR-RFLP analysis of glutamate dehydrogenase (gdh) locus was used to identify the genotypes found in cattle. In this method, ۴۳۲ bp expected size was amplified and then specific restriction NlaIV enzyme was used for subspecies detection. Totally, ۲۳ (۹.۳۴%) specimens were microscopically positive for giardiacyst out of ۲۴۶ examined samples. The PCR-RFLP analysis revealed that ۱۹ samples (۸۲.۶۰%) have the genotype E and ۴ samples (۱۷.۳۹%) belong to the subgroup AI. Our findings indicated that G. duodenalis infection is prevalent in cattle of Urmia and the non-zoonotic genotype E predominates in cattle in this region.

نویسندگان

Farnaz Malekifard

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Minoo Ahmadpour

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

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