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مقالات فارسیISIکنفرانسهاژورنالها

Livestock, pets and humans as carriers of methicillin-resistant Staphylococcus aureus and comparative evaluation of two PCR protocols for detection

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 7
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 55

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https://civilica.com/doc/1818640

شناسه ملی سند علمی:

JR_VRFAN-14-7_001

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Staphylococcus aureus are Gram positive bacteria known to acquire antibiotic resistance rapidly and pose a major challenge to clinicians worldwide. Infections by methicillin resistant Staphylococcus aureus (MRSA) are usually associated with increased mortality and prolonging of treatment. Samples (n = ۷۰۶) from diverse sources (livestock, pets, animal handlers, human hospital) were collected and screened for the presence of MRSA by phenotypic and genotypic methods. The incidence of Staphylococcus aureus was greater in goats (۴۲.۰۰%; ۲۸.۲۰ - ۵۶.۸۰%, confidence interval [CI] ۹۵.۰۰%) followed by cattle (۱۳.۵۰%; ۹.۲۰ - ۱۸.۸۰%, CI ۹۵.۰۰%), humans (۱۲.۹۰%; ۹.۳۰ - ۱۷.۴۰%, CI ۹۵.۰۰%) and dogs (۱۲.۹۰%; ۸.۱۰ - ۱۹.۲۰%, CI ۹۵.۰۰%). Significantly higher incidence of MRSA was observed in dogs (۶۵.۰۰%; ۴۰.۸۰ - ۸۴.۶۰%, CI ۹۵.۰۰%), compared to other hosts namely cattle (۴۸.۰۰%; ۲۶.۵۰ - ۶۴.۳۰%, CI ۹۵.۰۰%), humans (۳۵.۰۰%; ۲۰.۲۰ - ۵۲.۵۰%, CI ۹۵.۰۰%) and goats (۱۰.۰۰%; ۱.۲۰ - ۳۰.۴۰%, CI ۹۵.۰۰%). All the S. aureus isolates were further screened for thermostable nuclease (nuc gene) by polymerase chain reaction (PCR). The incidence of nuc gene in cattle, dog, goat and human were found to be ۳.۳۰% (۱.۳۰ - ۶.۶۰%, CI ۹۵.۰۰%), ۵.۲۰% (۲.۳۰ - ۹.۹۰%, CI ۹۵.۰۰%), ۲۸.۰۰% (۱۶.۲۰ - ۴۲.۵۰%, CI ۹۵.۰۰%) and ۹.۱۰% (۶.۰۰ - ۱۳.۰۰%, CI ۹۵.۰۰%), respectively. Comparative evaluation of two PCR primers (mecA-۱۶۲ and mecA-۳۱۰) indicated the former one as more rational choice for detection of MRSA. Overall, the results of our study indicated possible risk of zoonotic transmission of MRSA from canines.

کلیدواژه ها:

Cefoxitin ، mecA ، MRSA ، nuc

نویسندگان

Dharitree Sonowal

Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Sandeep Ghatak

Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India

Acheenta Barua

Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Srinivas Kandhan

Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India

Razibuddin Hazarika

Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Arnab Sen

Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India

Samir Das

Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India

Sarat Sonowal

Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Rajeev Sharma

Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Shantanu Tamuly

Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

Chimanjita Phukan

Department of Microbiology, Gauhati Medical College, Srimanta Sankaradeva University of Health Sciences, Guwahati, India

Ajanta Sharma

Department of Microbiology, Gauhati Medical College, Srimanta Sankaradeva University of Health Sciences, Guwahati, India

Poznur Hussain

Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India

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