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Effect of bovine viral diarrhea virus biotypes exposure on bovine gametes in early embryonic development in vitro

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 4
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 57

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https://civilica.com/doc/1818661

شناسه ملی سند علمی:

JR_VRFAN-14-4_005

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Bovine viral diarrhea virus (BVDV) is an important viral agent causing reproductive failure in cattle. The objectives of the current study were to investigate the interaction between two BVDV biotypes, cytopathic (CP) and Non-cytopathic (NCP) and bovine gametes during in vitro fertilization (IVF) processing, the existence of the virus within embryonic cells and early embryonic development rates. Sperm and ova were exposed separately to CP and NCP BVDV at two concentrations of ۱۰۴.۵ and ۱۰۵.۵ tissue culture infectious dose ۵۰.۰۰% (TCID۵۰) mL-۱ prior to IVF, respectively. After five days post-IVF, early embryonic development rates of infected groups were assessed. Several embryos of each group, normal and degenerated, were selected for a viral assay using reverse transcription polymerase chain reaction technique. The result showed that the early embryonic development rates were decreased in treatment groups. The rates in the CP groups were lower than the NCP groups. In the CP groups, the proportions were, respectively, ۱۰.۰۰, ۶.۰۰ and ۱۱.۰۰, and ۶.۰۰% in the infected sperm and oocyte groups (۱۰۴.۵ and ۱۰۵.۵ TCID۵۰ mL-۱) that were higher than ۵۰.۰۰% in the control group. In NCP groups, the rates were, respectively, ۲۵.۰۰, ۱۸.۰۰ and ۲۴.۰۰, and ۲۱.۰۰% in the infected groups compared to ۴۸.۰۰% in the control group. In the CP groups, no BVDV was detected in normal embryos, whereas, all degenerated embryos were completely virus-positive. In the NCP groups, the virus was detected in both normal and degenerated embryos. In conclusion, this study supported detrimental impacts of CP and NCP BVDV on early embryonic development and the role of sperm and the zona pellucida layer as carriers of the virus.

کلیدواژه ها:

Bovine viral diarrhea virus ، Cytopathic ، Early embryonic development ، In vitro fertilization ، Non-cytopathic

نویسندگان

Aidin Rahim Tayefeh

Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Massoud Talebkhan Garoussi

Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Farid Heidari

Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Mehran Bakhshesh

Department of Animal Virology, Research and Diagnosis, Razi Vaccine and Serum Research Institute (AREEO), Karaj, Iran

Abulfazl Shirazi

Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Maryam Vahidi

Department of Animal Biotechnology, Faculty of Agriculture Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

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