Prevalence, virulence factors, and antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli isolated from broiler chickens in Egypt

سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 39

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شناسه ملی سند علمی:

JR_VRFAN-14-3_003

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Pathogenic Escherichia coli is one of the world’s most important zoonotic foodborne pathogens and poses a serious threat to public health. We examined the prevalence, virulence genes, and antibiotic resistance profile of Shiga toxin (Stx)-producing E. coli (STEC) isolated from broiler chickens in the Kafr El-Sheikh governorate, Egypt. A total of ۴۱۰ samples (۲۳۰ cloacal swabs, ۱۸۰ internal organs) were collected to isolate E. coli. A total of ۲۹ (۷.۰۷%) E. coli isolates were recovered and identified, and ۱۸ of them harbored Stx genes (stx). Out of ۱۸ isolates, five (۱۷.۲۴%) carried the stx۱ gene, five (۱۷.۲۴%) carried the stx۲ gene, four (۱۳.۷۹%) carried both stx۱ and stx۲ genes, and four (۱۳.۷۹%) carried stx۱, stx۲, and eaeA genes. Overall, complete antibiotic resistance was observed against amoxicillin, ampicillin, cefpodoxime, and cefoperazone; high resistance was observed against ampicillin/sulbactam, nalidixic acid, cefuroxime, aztreonam, ciprofloxacin, ceftriaxone, chloramphenicol, sulfamethoxazole/ trimethoprim, and ceftazidime; moderate resistance against gentamicin; low resistance against cefoxitin; lower resistance was detected against norfloxacin, cefotetan, and amikacin; and the lowest resistance against imipenem. All E. coli isolates demonstrated multidrug resistance against at least four antibiotic classes. Out of ۲۹ E. coli isolates, STEC accounted for ۱۸ isolates, of which the O۷۸, O۲۶:H۱۱, O۱۲۸:H۲, O۱:H۷, O۱۱۹:H۶, and O۹۱:H۲۱ serogroups were predominant. All E. coli isolates were multidrug resistant and therefore pose a potential public health concern as these virulent, resistant strains may spread to humans. Thus, high levels of hygiene and biosecurity are required by chicken handlers to decrease the danger of infection spreading to humans.

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نویسندگان

Ibtisam Hasona

Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt

Salwa Helmy

Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt

Adel El Gamal

Unit of Bacteriology, Animal Health Research Institute, Kafr El-Sheikh Branch, Agriculture Research Center (ARC), Egypt

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