Phylogenetic analysis of canine parvovirus isolates from west Mediterranean region of Türkiye
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 3
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 45
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-14-3_001
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Canine parvovirus type ۲ (CPV-۲) causes hemorrhagic enteritis, and is one of the most important and contagious pathogens of dogs. In this study, we aimed to determine the prevalence and antigenic variants of CPV enteritis in dogs. Fecal samples were collected from ۳۵ dogs with mucoid to hemorrhagic diarrhea in the Western Mediterranean region of Türkiye between October ۲۰۱۹ and March ۲۰۲۱. DNA was isolated from the samples and examined using PCR analysis. Twenty-eight out of ۳۵ dogs (۸۰.۰۰%) were detected to be positive for CPV. Of these, three had already been vaccinated. The partial VP۲ genes of ۱۵ CPV positive samples producing strong bands in agarose gels were sequenced. All strains were identified as CPV-۲b, and the amino acid changes were identified. Discriminative amino acid changes were detected for different amino acid positions clearly defining new CPV-۲b variants. Of the ۱۵ isolates, three had previously unreported synonymous mutations. Phylogenetic analysis indicated that the strains obtained in this study were closely related to isolates from the Mersin province of Türkiye, except for three isolates that had synonymous mutations and were located in a separate branch from the other CPV-۲b genetic variants previously detected in Mersin Province and Urfa Province in Türkiye. This study demonstrates the increase in the prevalence rates for CPV-۲b circulating in vaccinated and nonvaccinated dogs. Taking into account the data from phylogenetic trees which highlights differences between the vaccine strains and the isolates, re-designing immunization strategies needs necessary.
کلیدواژه ها:
نویسندگان
Sibel Hasircioğlu
Department of Virology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Türkiye
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :