Evaluating Oxidative Stress Condition in Human Bladder Cancer ۵۶۳۷ Cell Line upon Exposure to Silver Nanoparticles

Sajedeh Daei; Nasrin Ziamajidi; Roghayeh Abbasalipourkabir; Zeynab Aminzadeh; Mohammad Vahabirad

Evaluating Oxidative Stress Condition in Human Bladder Cancer ۵۶۳۷ Cell Line upon Exposure to Silver Nanoparticles

محل انتشار: مجله سرطان خاورمیانه، دوره: 14، شماره: 3

سال انتشار: 1402

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 75

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لینک ثابت به این مقاله:

https://civilica.com/doc/1819051

شناسه ملی سند علمی:

JR_MISJ-14-3_003

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Background: Bladder cancer (BC) is known as the most frequent neoplasm of the urinary system, whose prevalence has significantly increased over the past three decades. Successful treatment of BC is a highly challenging task. In this regard, several studies have demonstrated that increased level of oxidative stress may cause cancer cells death. Furthermore, silver nanoparticles (AgNPs) are recognized as one of the most widely used nanomaterials in cancer treatment. Herein, we evaluated the AgNPs-induced oxidative stress in BC ۵۶۳۷ cell line.Method: In the current experimental study, using colorimetric reactions, we assessed the levels of oxidative stress parameters, including malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), as well as the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) as antioxidant enzymes. Moreover, we performed the statistical analysis via One-way ANOVA and post-hoc Tukey tests to draw comparisons between the groups.Results: The results indicated an increased amount of TOS, MDA, and oxidative stress index. Nonetheless, there was a remarkable reduction in SOD, GPx, and CAT activities and TAC level in the AgNPs-exposed cells compared to the control untreated ones (P < ۰.۰۵).Conclusion: All in all, AgNPs have the potential to induce oxidative stress in ۵۶۳۷ cells. We thus concluded that AgNPs can be chosen as an antitumor agent for future investigations to treat BC.

کلیدواژه ها:

Metal Nanoparticles ، Silver ، Oxidative stress ، Urinary bladder ، Cell Line

نویسندگان

Sajedeh Daei