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مقالات فارسیISIکنفرانسهاژورنالها

Combined Effects of Sodium Valproate and Lithium Chloride on Inducing Apoptosis and Inhibiting Invasion of PC۳ Cells

محل انتشار: مجله سرطان خاورمیانه، دوره: 14، شماره: 2
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 40

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1819069

شناسه ملی سند علمی:

JR_MISJ-14-2_005

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Background: In this study, we aimed to investigate the combined effect of sodium valproate (SV) and lithium (Li) against viability, migration, and invasion of prostate cancer cell line.Method: In this in vitro study, PC۳ cells were treated with different concentrations of SV (۲.۵, ۵.۰, and ۱۰ μM) and Li (۲.۵, ۵.۰, and ۱۰ mM) either alone or in combination. Using the MTT test and annexin V/۷ADD flow-cytometry, cell viability and apoptosis were assessed. Transwell chamber test was used to assess PC۳ cells' invasion and migration.Results: SV and Li alone had no significant effects on PC۳ cell viability. However, the combination of SV and Li in all tested concentrations decreased the viability of PC۳ cells in a dose dependent manner (P < ۰.۰۰۱). The combination of SV and Li (۵.۰ μM + ۵.۰ mM) increased apoptosis of PC۳ cells compared with the control group (P = ۰.۰۰۳). Transwell assay showed that combination of SV with Li (۵.۰ μM + ۵.۰ mM) reduced the migration and invasion of PC۳ cells significantly. The lack of a significant difference between the predicted and observed fractional inhibition for the effects of SV+Li suggests that SV and Li may have additive effects on lowering PC۳ cell viability and invasiveness.Conclusion: These findings show that combined low doses of SV and Li could decrease the viability and invasiveness of the PC۳ cells; therefore, it can be considered as a new strategy for the treatment of prostate cancer. However, further in vivo studies are required to confirm the results of this study.

کلیدواژه ها:

Prostatic Neoplasms ، Valproic acid ، Lithium ، Combined modality therapy ، apoptosis

نویسندگان

Mohammad Ali Takhshid

Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Samaneh Davoudzadeh

School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

Ghazal Noohi

School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

Samaneh Naderi

Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Bahareh Zamani

Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

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