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مقالات فارسیISIکنفرانسهاژورنالها

In Vitro Radiosensitization of T۴۷D and MDA-MB-۲۳۱ Breast Cancer Cells with the Neoflavonoid Dalbergin

محل انتشار: مجله سرطان خاورمیانه، دوره: 14، شماره: 2
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 61

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لینک ثابت به این مقاله:
https://civilica.com/doc/1819072

شناسه ملی سند علمی:

JR_MISJ-14-2_002

تاریخ نمایه سازی: 25 آبان 1402

چکیده مقاله:

Background: Radiotherapy is a frequently used therapeutic modality for breast cancer. Dalbergin, a natural antioxidant, inhibits carcinogens and tumor progression. In the present study, we investigated the effect of Dalbergin on the response of T۴۷D and MDA-MB-۲۳۱ breast cancer cell lines to ionizing radiation.Method: In this experimental in vitro study, doubling time of T۴۷D and MDAMB- ۲۳۱ were obtained from the growth curve. The cytotoxic effect of Dalbergin on T۴۷D and MDA-MB-۲۳۱ breast cancer cells were estimated via MTT assay. To determine the clonogenic ability, we treated T۴۷D and MDA-MB-۲۳۱ with Dalbergin for ۴۸ h prior to irradiation, subsequent to which a colony assay was performed. Real-time polymerase chain reaction was employed to determine the gene expression level.Results: Dalbergin inhibited proliferation of T۴۷D and MDA-MB-۲۳۱ in a time and concentration-dependent manner. Additionally, the most appropriate time for the treatment of these types of cancer cells was found to be ۴۸ h and the drug's concentration in both cell lines was different. The IC۵۰ values of T۴۷D and MDA-MB-۲۳۱ cells were ۰.۰۰۱ and ۰.۰۰۰۱ μM, respectively. Moreover, this drug radiaosensitizes both cell lines effectively compared with the radiation only. Finally, the gene expression level of p۵۳, Bcl-۲, and STAT۳ were investigated in cancer cells.Conclusion: Dalbergin showed apoptotic effects probably through the STAT/p۵۳ signaling pathway. Therefore, Dalbergin could be considered as a radiosensitizer and its effects may be owing to increased cell death.

کلیدواژه ها:

Dalbergin ، Cells ، X-rays ، Apoptotic ، Cell death

نویسندگان

Fereshte Mahdizade Valojerdi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Bahram Goliaei

Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Nakisa Rezakhani

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Alireza Nikoofar

Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran, Iran

Hoda Keshmiri Neghab

Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran

Mohammad Hasan Soheilifar

Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran

Bahareh Bigdeli

Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

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