Anti-Cancer Properties of Nicotinic Cid-Alpha Linolenic Acid Derivative on A۳۷۵ Melanoma Cell Line: Assessment of Apoptosis and WNT Signaling Pathways
محل انتشار: مجله سرطان خاورمیانه، دوره: 13، شماره: 4
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 54
فایل این مقاله در 14 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_MISJ-13-4_004
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Background: Malignant melanoma is an aggressive skin cancer whose survival rate is extremely low. Commencing apoptosis is believed to be a significant issue in cancer treatment and targeting the apoptosis and WNT signaling pathways, which is probably a potentially successful strategy to overcome tumor plasticity in melanoma.Method: We conducted the present in vitro study to investigate antiproliferative and apoptotic effects of Nic-ALA, as a new compound, on A۳۷۵ melanoma cell line using MTT assay and flow cytometry, respectively. The gene expression profiles of the cancer cells were obtained for Bcl-۲ and BAX as the main genes of the apoptosis signaling pathway and WIF۱ and beta-catenin genes from the WNT signaling pathway with qRT-PCR.Results: Nic-ALA’s cytotoxicity on A۳۷۵ melanoma cell line from MTT assay was obtained with IC۵۰ ۱۶۶.۷, ۱۴۴.۲, and ۱۴۶.۱μM. This novel derivative induced ۱۱.۳, ۴۶.۱, and ۸۵.۷% of apoptosis in ۲۴, ۴۸, and ۷۲h time points, respectively. In the treated cells, the expression of BAX, beta-catenin, and WIF۱ genes increased, while the expression of Bcl-۲ decreased significantly at ۲۰۰μM concentration and the treated times of ۴۸ and ۷۲h.Conclusion: The antiproliferation of Nic-ALA at a lower value than what we found in nicotinic acid alone represented the higher bioavailability and transport efficiency of this novel derivative through A۳۷۵ melanoma cell line. Its antipoetic effects were obtained by increasing the apoptosis rate and expression of the Bax gene and reducing Bcl-۲ gene expression. Upregulation of WIF۱ and beta-catenin in the WNT signaling pathway emphasized Nic-ALA’s anticancer effect on A۳۷۵ melanoma cell line.
کلیدواژه ها:
Nicotinic acid-alpha linolenic acid ، Wnt signaling pathway ، WIF۱ ، beta-catenin ، apoptosis ، Melanoma
نویسندگان
Hamide Malikhan
Department of Genetics, College of Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Elham Siasi Torbati
Department of Genetics, College of Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Ahmad Majd
Department of Genetics, College of Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Nematollah Gheibi
Cellular and Molecular Research Center, Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :