Serine proteases activity in miracidia of Fasciola hepatica and effects of chemical and herbal inhibitors
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 38
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شناسه ملی سند علمی:
JR_VRFAN-12-3_005
تاریخ نمایه سازی: 27 آبان 1402
چکیده مقاله:
Fasciolosis is a zoonotic parasitic disease caused by the trematode Fasciola hepatica. The proteases are essential for the survival of parasites. The present study was aimed to determine serine proteases activities in miracidia of F. hepatica and evaluate the effects of pH and different inhibitors on the serine proteases activities. Adult F. hepatica helminths were removed from naturally infected livers of the slaughtered cattle and crushed. The eggs were incubated at ۲۸.۰۰ ˚C for ۱۶ days. The released miracidia were homogenized and total proteolytic activity of the extract of miracidia at different pH values were evaluated. Serine proteases activities were determined using specific substrates. The inhibitory effects of chemical and herbal inhibitors on the enzymes were also assessed. The extract of miracidia hydrolyzed azocasein with optimum activity at pH ۸.۰۰. The optimum pH effect on serine proteases activities was found at alkaline pH. Phenylmethylsulfonyl fluoride and Bowman-Birk inhibitors inhibited and decreased the proteases activities in the miracidia extract. It was concluded that there were proteases activities in miracidia of F. hepatica which were inhibited by chemical and herbal inhibitors.
کلیدواژه ها:
نویسندگان
Leila Kianifard
PhD Candidate, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mohammad Yakhchali
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mehdi Imani
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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