Pro-oxidant and degenerative effects of haloperidol under inflammatory conditions in rat; the involvement of SIRT۱ and NF-κB signaling pathways

Saman Bahrambeigi; Mahsa Khatamnezhad; Siamak Asri-Rezaei; Bahram Dalir-Naghadeh; Shahram Javadi; Navideh Mirzakhani

Pro-oxidant and degenerative effects of haloperidol under inflammatory conditions in rat; the involvement of SIRT۱ and NF-κB signaling pathways

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 2

سال انتشار: 1400

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 64

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لینک ثابت به این مقاله:

https://civilica.com/doc/1820647

شناسه ملی سند علمی:

JR_VRFAN-12-2_006

تاریخ نمایه سازی: 27 آبان 1402

چکیده مقاله:

The present study was conducted to evaluate the effects of different doses of haloperidol (HP) on induction of oxidative stress in blood and liver cell degeneration in comparison with influences of HP pre-treatment on inflammatory process induced by intraperitoneal (IP) administration of lipopolysaccharide (LPS). One hundred twenty male albino Wistar rats were randomly divided into eight groups (۱۵ in each), including: Control group, LPS group, three groups as HP administration in three divided doses (۰.۵۰, ۱.۰۰ and ۲.۰۰ mg kg-۱), and three treatment groups that HP was administered in three doses (۰.۵۰, ۱.۰۰ and ۲.۰۰ mg kg-۱) prior to LPS administration.Concentrations of malondialdehyde, activities of antioxidant enzymes including glutathione peroxidase, superoxide dismutase and also the levels of tumor necrosis factor-alpha and interleukin ۱-beta were measured in blood and serum. In addition to liver histopathological changes evaluation, hepatic silent information regulator of transcription ۱ (SIRT۱) and phosphorylated-nuclear factor-κB (p-NF-κB) levels were quantitated. Our findings indicated that sole administration of HP (particularly higher doses) can induce oxidative stress in blood and cell degeneration in liver, while it can attenuate inflammatory process induced by LPS administration presumably via SIRT۱ up-regulation and preventing the induction of p-NF-κB. The oxidative and degenerative effects of HP and its impact on inflammatory status were completely dose- dependent according to our results. The possible anti-inflammatory effects of HP may affect reparative mechanisms and hepatic cell degeneration. However, the influences of HP on immune system need further investigations and its higher doses should be administered cautiously especially in patients with immune system dysfunctions.

کلیدواژه ها:

Haloperidol ، Lipopolysaccharide ، NF-κB ، Oxidative stress ، SIRT۱

نویسندگان

Saman Bahrambeigi