Conventional histomorphometry and fast free of acrylamide clearing tissue visualization of sciatic nerve in chicken (Gallus domesticus)
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 69
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شناسه ملی سند علمی:
JR_VRFAN-12-2_005
تاریخ نمایه سازی: 27 آبان 1402
چکیده مقاله:
Histomorphometry and use of the fast free of acrylamide clearing tissue (FACT) protocol were studied on the sciatic nerve in chicken (Gallus domesticus). In the first part of the study, the sciatic nerves of ۲۰ chickens of four age groups (۷, ۱۴, ۲۶ and ۴۰ days) were studied (n=۵ birds per age class). Their sciatic nerve samples were stained with Hematoxylin and Eosin and Masson's trichrome and were histomorphometrically evaluated. In the second part of the study, FACT protocol was applied on the sciatic nerve of a ۲۶ days old chicken. After clearing of ۱.۰۰ mm-thick sciatic nerve sections, they were immunolabelled using Hoechst for nuclei staining and recorded by a Z-stack motorized fluorescent microscope. In the conventional histo-morphometry, the epineurium, perineurium and endoneurium were thicker and the nerve bundle diameter was bigger in the left sciatic nerve of chicken of all age groups compared to the right sciatic nerve. On the contrary, the axon diameter and the myelinated nerve fiber diameter were bigger, the myelin sheath was thicker, the nodes of Ranvier intervals were higher and the density of myelinated nerve fibers was also higher in the right sciatic nerve compared to the left one. In conclusion, histomorphometric parameters in the left and right sciatic nerve during chicken growth were significantly different. Furthermore, the FACT protocol could be used for the ۳D imaging of the chicken sciatic nerve and its immunostained evaluation.
کلیدواژه ها:
نویسندگان
Abdolrasoul Malekpour
DVM Graduate, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Farhad Rahmanifar
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
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