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مقالات فارسیISIکنفرانسهاژورنالها

Viability of in vitro preserved sperm of Harris’s hawk (Parabuteo unicinctus)

محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1820652

شناسه ملی سند علمی:

JR_VRFAN-12-2_001

تاریخ نمایه سازی: 27 آبان 1402

چکیده مقاله:

Harris hawks (Parabuteo unicinctus) are the raptors with more anthropogenic use, which makes it necessary to improve their reproduction either natural or assisted for sustainable use even though they are not endangered. The aim of this study was to determine fresh and thawed P. unicinctus sperm capacitation and acrosome reaction parameters as indicators of sperm viability in ejaculates of birds with injuries. A total of ۳۰ ejaculates were analyzed as fresh and thawed samples. Basic sperm evaluations as well as in vitro acrosomal reaction capability through the presence and distribution of Ca۲+ and N-acetylglucosamine and sialic acid using chlortetracycline and agglutinin-fluorescein isothiocyanate Triticum vulgaris were performed,respectively. Tagged samples were observed using epifluorescence microscopy. The results indicated ۳۰.۰۰% decrease in sperm viability following freeze-thaw, while rate of capacitated sperms (۱۰.۰۰%) and the percentage of sperm with acrosomal reaction (۴۰.۰۰%) were increased. The results of the experiment demonstrated that the post-thaw viability of the obtained sperm may be an alternative for use in artificial insemination.

کلیدواژه ها:

Acrosome ، Cryopreservation ، glucosamine ، Membrane

نویسندگان

Cuauhtémoc Cruz-Valencia

Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico

José Antonio Herrera-Barragán

Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Fernando Gual-Sill

Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Juán José Pérez-Rivero

Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Zulma América Soto-Guerrero

Secretary of Environment, Bird of Prey Rehabilitation Center, Aguascalientes, Mexico

Fernanda Rodriguez-Hernández

Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Juán Gabriel Rivera-Martínez

Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico

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