Anti-Cancer Activities of Magnetic DNASilver Nanoclusters in Breast Cancer

Background: Recent advances in nanotechnology have vastlychanged the diagnosis, treatment and prevention of various diseasesin all aspects of life. Among noble metals, silver nanoclusters(AgNC) are particularly attractive due to their uniquephysical properties such as strong luminescence and very smallsize. Among the various nanoparticles, magnetic nanoparticles(MNPs) offer a wide range of applications in life sciences.According to the literature, the absorption of MNPs by cellsmay depend on the coating of MNPs. Biologically synthesizedMNPs probably have a good potential in carrying DNA-AgNCas well as absorption by cells.Materials and Methods: The present work is the successfulsynthesis of biosynthesized iron oxide nanoparticles (Fe۳O۴-NPs) using the non-toxic extract of Rumex Crispus (R. Crispus)leaves, as a suitable carrier for DNA-AgNC and investigatingits use in cell viability and cytotoxicity. Analysis of various synthesizedstructures was characterized by UV-Vis and DLS.Results: DLS analysis showed that the size distribution ofMNP is ۱۳۲nm and zeta potential is ۶۹.۴ mV. These nanoparticleshave the maximum absorption at ۴۰۲ nm, the DNA-AgNChas the maximum absorption at ۴۳۵ nm, and the MNP@DNAAgNC complex has the maximum absorption at ۳۹۳ nm. Theresults of the cell culture showed that the MNP@DNA AgNCcomplex at the highest concentration had the highest cell viabilityand the lowest cytotoxicity, and at the lowest concentration,it had the highest cytotoxicity and the lowest viability. Therefore,from the above results, we find that higher doses causecell resistance, but lower doses cause cytotoxicity. Also, bycomparing the level of cytotoxicity and cell viability betweenthe three treatments including MNP, DNA-AgNC, and MNP@DNA AgNC, it was concluded that MNP carrying DNA AgNCcauses more toxicity to cancer cells and changes their viability.Conclusion: The results of the behavior of this complex showthat it has potential applications for a gene and drug deliverysystem, photothermal and combination therapy.