Background: Cervix uteri cancer is predicted to be the fourthleading cause of cancer-related illness and death among womenglobally. Several studies have demonstrated that non-codingRNA (ncRNA), specifically long non-coding RNA (lncRNA),is involved in the development of tumors. Our aim was to examinethe expression of lncRNAs and mRNAs in cervix utericancer and normal samples in order to pinpoint diagnosis-relatedRNAs and investigate potential molecular pathways.Materials and Methods: The gene-expression data (FPKMvalues) in tumor and normal tissues of patients with cervix utericancer through RNA sequencing were obtained from GDCwebsite (https://portal.gdc.cancer.gov/). We used the TCGAbiolinksR package to identified significant differentially expressedgenes (DEGs) that met the screening criteria | [log FC]| ≥ ۲, adjusted p-value < ۰.۰۵. We also identified differentiallyexpressed lncRNAs using the same methods. Finally, we conductedGene Ontology (GO), Kyoto Encyclopedia of Genesand Genomes (KEGG), gene set enrichment, and protein-proteininteraction (PPI) network analyses to find hub lncRNAsand mRNA.Results: We identified ۱۴۹۰ DEGs, of which ۷۹۱ were significantlyupregulated and ۶۹۹ were significantly downregulated.These DEGs were enriched in four KEGG pathways, namely metabolism of xenobiotics by cytochrome P۴۵۰, retinol metabolism,neuroactive ligand-receptor interaction, and drug metabolismpathways. Among the identified DEGs, OLFM۳, KRT۲۶,KRTAP۳-۳, AMELX, and TMPRSS۱۵ were the most upregulatedgenes, while REG۱A, SI, MUC۵B, REG۳A, and UGT۲A۳were the most downregulated. Furthermore, we identified ۱۱۴۴differentially expressed lncRNAs that were closely related tocervix uteri cancer. By intersecting the differentially expressedlncRNAs with hub lncRNAs, we identified LINC۰۰۹۶۷ as themost upregulated lncRNA and LINC۰۱۵۴۱ as the most downregulatedlncRNA.Conclusion: In Conclusion: , this study suggested significantlncRNAs as biomarkers associated with
cervix uteri cancer andidentified crucial genes and pathways for better diagnosis andtreatment. However, further experimental validation is requiredto confirm the results and comprehend the underlying molecularmechanisms.