Identification of the interaction of IL۲۴with hsa-miR-۱۰a-۵p and AIRN, and rs۷۵۷۶۲۰۱۸ as potentialbiomarkers in tongue neoplasm development: Bioinformaticapproach

Background: One of the most common areas of head and neckcancer is the tongue. In this study, we used Microarray datato explore gene expression in tongue carcinoma and normaltongue tissues derived from patients to identify novel biomarkersfor diagnosis.Materials and Methods: First, we obtained GSE۳۴۱۰۶ fromthe Gene Expression Omnibus (GEO) database and analyzedit using GEO۲R online software to identify differentially expressedgenes (DEGs) in tongue carcinoma and non-malignantcontrol samples. We confirmed our previous results by usingthe GEPIA۲ and ENCORI databases. Afterward, we used themiRWALK ۲.۰ database to find mRNA-miRNA interactionsand lncRRIsearch to find mRNA-lncRNA interactions. Singlenucleotide polymorphisms (SNPs) were acquired from the db-SNP and SIFT and HOPE databases.Results: Microarray analysis revealed that IL۲۴ was significantlyoverexpressed in malignant tissues compared to controlsunder these conditions (logFC= ۴.۵۵۲۳۵۶۱۵, adjusted p-value =۰.۰۰۰۰۰۰۰۷۲۲). After obtaining miRNAs interacting with IL۲۴from the miRWALK database and performing correlation analysison ENCORI, we chose hsa-miR-۱۰a-۵p (r=-۰.۱۸۳, p value=۴.۱۳e-۰۵). Based on the lncRRIsearch and lncRNADiseasedatabases, as is seen in other types of cancers, a lncRNA calledAIRN is predicted to influence tongue neoplasms by targetingIL۲۴. rs۷۵۷۶۲۰۱۸ is one of the most deleterious mutations inthe coding region of IL۲۴. In this SNP, Leucine mutates intoproline at position ۱۰. The mutation is located within the signalpeptide, and it likely disturbs the signal peptide's recognition.As this mutation occurs in a highly conserved region, it maydamage the protein.Conclusion: Several pieces of evidence support the correlationof IL۲۴ with has-miR-۱۰a-۵p, IL۲۴ interaction with AIRN,and their impact on the promotion of tongue cancer. Moreover,rs۷۵۷۶۲۰۱۸ can promote the development of tongue cancer bymodifying the folding and interactions of this protein.