Evaluation of Genetic Diversity among South Tunisian Pomegranate (Punica granatum L.) Accessions Using Fruit Traits and RAPD Markers

Morphological and RAPD markers were used to investigate the genetic diversity among ۲۱ accessions of pomegranate originating from South Eastern Tunisia. Thirteen morphological traits were studied and results showed significant differences for all morphological characters (P< ۰.۰۰۱). Clustering based on fruit traits, using Ward’s method, divided the accessions into three main groups. In RAPD analysis, ۶ out of ۱۵ employed random primers showed good amplification and polymorphism on pomegranate samples with a total of ۶۳ bands, of which ۵۶ were polymorphic. The lowest percentage of polymorphism (۵۰%) was observed with TIBMBA-۰۳ while the highest (۵۰%) was observed with primer TIBMBB-۰۳. According to Jaccard coefficient, the lowest (۰.۲۹) and highest (۰.۹۴) similarities were detected between genotypes. UPGMA clustering based on data from polymorphic RAPD bands resulted in three clusters at a similarity of ۰.۴۶. The Stress value for the nonmetric multidimensional scaling plot was ۰.۰۷۱, showing an excellent representation of the data. The comparison between groupings based on the fruit traits and RAPD data did not produce a significant correlation (r= -۰.۰۹). Using a stepwise linear regression, significant regressions were found between ۱۳ morphological traits and ۶۳ molecular markers revealing association between RAPD molecular markers and some traits.