Identification of key genes involved in heat stress response in Brassica napus L.: reconstruction of gene networks, hub genes, and promoter analysis
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 76
فایل این مقاله در 16 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJGPB-11-1_006
تاریخ نمایه سازی: 4 آذر 1402
چکیده مقاله:
Brassica napus is a versatile crop with oil and protein-rich seeds, used in food, industry, medicine, and animal feed. However, heat stress limits its productivity, making it essential to identify genes and pathways involved in stress response. We analyzed differentially expressed genes (DEGs) in B. napus under heat stress using bioinformatics tools to identify key genes and pathways. Firstly, DEGs were analyzed for gene interactions using the STRING database and visualized using Cytoscape. To identify key genes involved in heat stress response in B. napus L., we employed the CytoHubba tool for hub gene identification. Additionally, we conducted Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses to gain insights into the functional roles and potential biological pathways associated with these genes. We also used MEME Suite to analyze the promoter regions of hub genes. Our results showed decreased activity of the b۶-f complex, a key component of the electron transport chain, under heat stress. We also identified significantly enriched calcium transporter ATPase and heat shock protein family (HSP۲۰). KEGG and cluster analyses highlighted the importance of membrane lipids, galactose metabolism, and protein processing in the endoplasmic reticulum in stress and signal transduction. Our study provided key genes, including transcription factors and chaperones for developing heat-resistant plants via genetic modification. However, these promising results were obtained through rigorous bioinformatics analysis and require further validation using experimental approaches, such as gene editing, phenotypic characterization, and field trials.
کلیدواژه ها:
نویسندگان
مینا مکوندی
Department of Agricultural Biotechnology, Payame Noor University (PNU), Isfahan, Iran.
ابوذر قربانی
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj, Iran.
مهسا رستمی
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj, Iran.
عاطفه رستمی
Department of Biology, Faculty of Science, University of Zanjan, Zanjan, Iran.
علی اکبر قاسمی سلوکلویی
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :