Porphyrin Molecules Decorated on Metal-Organic Frameworks forMulti-Functional Biomedical applications

Metal–organic frameworks (MOFs) have been widely used as porous nanomaterials fordifferent applications ranging from industrial to biomedicals ([۱]). An unpredictable one‐potmethod is introduced to synthesize NH۲‐MIL‐۵۳ assisted by high‐gravity in a greener media forthe first time. Then, porphyrins were deployed to adorn the surface of MOF to increase thesensitivity of the prepared nanocomposite to the genetic materials and in‐situ cellular proteinstructures. The hydrogen bond formation between genetic domains and the porphyrin’ nitrogen aswell as the surface hydroxyl groups is equally probable and could be considered a milestone inchemical physics and physical chemistry for biomedical applications ([۲]). In this context, the roleof incorporating different forms of porphyrins, their relationship with the final surfacemorphology, and their drug/gene loading efficiency were investigated to provide a predictablepattern in regard to the previous works. The conceptual phenomenon was optimized to increasethe interactions between the biomolecules and the substrate by reaching the limit of detection to۱۰ pM for the Anti‐cas۹ protein, ۲۰ pM for the single‐stranded DNA (ssDNA), below ۱۰ pM forthe single guide RNA (sgRNA) and also around ۱۰ nM for recombinant SARS‐CoV‐۲ spikeantigen. Also, the MTT assay showed acceptable relative cell viability of more than ۸۵% in mostcases, even by increasing the dose of the prepared nanostructures ([۳]).