Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album

سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 90

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شناسه ملی سند علمی:

JR_JJMPB-2-1_012

تاریخ نمایه سازی: 6 آذر 1402

چکیده مقاله:

The natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. In order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect of different concentrations of α-Naphthalene acetic acid (NAA) (۰, ۰.۴ and ۱ mg/l), Kinetin (Kin) (۰, ۰.۲ and ۰.۴ mg/l) and combinations of NAA and Kin [(۰.۴ and ۰.۲), (۰.۴ and ۰.۴), (۱ and ۰.۲) and (۱ and ۰.۴) mg/l] on growth and accumulation of podophyllotoxin in callus, cell culture and in vitro propagated plantlets of Linum album was evaluated. The highest growth and accumulation of podophyllotoxin in callus culture was obtained when it grown under dark condition in comparison to photoperiod. The biomass of cell and callus do not changed under various hormone concentrations. Content of podophyllotoxin was enhanced in cell and callus cultures supplemented with NAA ۱ mg/l + Kin ۰.۴ mg/l. The experiments demonstrated Kin ۰.۲ or ۰.۴ mg/l stimulated multiplication and production of podophyllotoxin in shoot culture.

نویسندگان

Safieh Fakhari

Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, P.O. Box ۱۴۱۱۵-۱۵۴ Iran

Mozafar Sharifi

Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, P.O. Box ۱۴۱۱۵-۱۵۴ Iran

Morteza Yousefzadi

Department of Biology, Faculty of Basic Sciences, Hormozgan University, Bandar Abbas, P.O. Box ۳۹۹۵ Bandar Abbas, Iran

Elham Beshamgan

Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, P.O. Box ۱۴۱۱۵-۱۵۴ Iran

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