In vitro Response of Asparagus breslerianus to NaCl

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 65

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شناسه ملی سند علمی:

JR_JJMPB-6-2_006

تاریخ نمایه سازی: 6 آذر 1402

چکیده مقاله:

Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to ۰, ۲۱.۸۸, ۴۳.۷۶, ۶۵.۶۴, ۷۶.۵۸, ۸۷.۵۲, ۱۰۹.۴۰, ۱۳۱.۲۸, ۱۵۳.۱۶ and ۱۷۵.۰۴ mM NaCl in MS (Murashige and Skoog) basal  medium supplemented with ۰.۸۸ µM BA (۶-Benzylaminopurine) and ۱.۰۷ µM NAA (۱- Naphthalene acetic acid). According to results, friable and compact type, green and white green calli were obtained up to ۸۷.۵۲ mM NaCl. Soft type, yellow and cream calli were observed with increasing salinity further ۱۰۹.۴ mM NaCl. Calli growth and plantlets regeneration were high in media up to ۱۰۹.۴ mM NaCl. In term of mineral accumulation, sodium content increased with an increase in NaCl levels. It indicates that A. breslerianus calli could acclimatize to salt stress by high osmotic adjustment. These suggested that, under in vitro salt-induced osmotic stress, A. breslerianus is reflected as salt resistant which tolerate NaCl up to ۱۰۹.۴ mM.

نویسندگان

Seyyed Javad Mousavizadeh

Department of Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Mohammad Reza Hassandokht

Department of Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abdolkarim Kashi

Department of Horticultural Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

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