Genetic Diversity Evaluation of Lemon balm (Melissa officinalis L.) Ecotypes Using Morphological Traits and Molecular Markers

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 68

فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_JJMPB-6-1_012

تاریخ نمایه سازی: 6 آذر 1402

چکیده مقاله:

Genetic diversity of ۱۲ lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created ۱۰۶ polymorphic bands among the studied ecotypes. An ISSR primer called UBC۸۱۳ with ۱۶ bands and UBC۸۱۱, UBC۸۱۵ and UBC۸۱۷ primers with ۱۵ bands had the highest number of polymorphic bands and UBC۸۲۵ with ۸ bands had the lowest number of polymorphic bands. In addition, ۱۰ RAPD primers, created ۱۲۷ polymorphic bands, among them OPA-۰۱with ۲۲ bands and BB۱۳ and OC۴primers with ۱۹ and ۱۸ bands had the highest number of polymorphic bands. In contrast, OS-۰۳and OB۲۰markers had the lowest number of polymorphic bands with ۱۴ and ۱۵ bands, respectively. Polymorphism information content (PIC) value ranged between ۰.۳۳ to ۰.۳۷ for ISSR markers and ۰.۳۱ to ۰.۳۷ for RAPD markers. Marker index (MI) value ranged between ۱.۱۱ to ۴.۳۸ for ISSR markers and ۱.۸۵ to ۵.۲۸ for RAPD markers. Cluster analysis using UPGMA method based on ISSR and RAPD markers results clustered the studied ecotypes into three and two different groups, respectively, by which the percentage of similarity of the two grouping method was about ۵۰%. Grouping the ecotypes based on both molecular markers and agronomic traits matched each other about ۴۰ to ۶۰%.

کلیدواژه ها:

نویسندگان

Mehdi Rahimi

Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

Mojtaba Kordrostami

Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Saeidi H, Rahiminejad MR, Heslop-Harrison J. Retroelement insertional polymorphisms, diversity ...
  • Sofalian O, Chaparzadeh N, Javanmard A, Hejazi M. Study the ...
  • Shyamalamma S, Chandra S, Hegde M, Naryanswamy P. Evaluation of ...
  • El Hadj Ali IB, Guetat A, Boussaid M. Genetic diversity ...
  • Belhassen E, Atlan A, Couvet D, Gouyon P, Quétier F. ...
  • Alamdary S, Safarnejad A, Rezaee M. Evaluation of genetic variation ...
  • Smolik M, Jadczak D, Korzeniewska S. Assessment of morphological and ...
  • Yavari A, Nazeri V, Sefidkon F, Zamani Z, Hassani M. ...
  • Danaeipour Z, Fotokian MH, Talei D. Genetic diversity in Melissa ...
  • Ghaffariyan S, Mohammadi SA, AhariZad S. Patterns of population diversity ...
  • Haidari P, Mehrabi AA, Nasrollah Nejad Ghomi AA. Genetic diversity ...
  • Noroozy S, Sharafi Y, Talei D, Naji AM (۲۰۱۶) Evaluation ...
  • Murray M, Thompson WF. Rapid isolation of high molecular weight ...
  • Kimura M, Crow JF. The number of alleles that can ...
  • Yeh F, Yang R-C, Boyle TJB, Ye Z-H, Mao JX. ...
  • Anderson JA, Churchill G, Autrique J, Tanksley S, Sorrells M. ...
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of ...
  • Nei M. Genetic distance between populations. Am Nat. ۱۹۷۲;۱۰۶:۲۸۳-۲۹۲ ...
  • Lewontin RC. The apportionment of human diversity. In: Dobzhansky T, ...
  • Rohlf F. NTSYS-pc numerical taxonomy and multivariate analysis system. Ver. ...
  • Fisher RA. The use of multiple measurements in taxonomic problems. ...
  • Solouki M, Mehdikhani H, Zeinali H, Emamjomeh A. Study of ...
  • Pirkhezri M, Hassani M, Hadian J. Genetic Diversity in Different ...
  • Heidary S, Marashi H, Farsi M, Mirshamsi Kakhki A. Assessment ...
  • Zhang J-T, Xu B, Li.M. Genetic diversity of populations of ...
  • نمایش کامل مراجع