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مقالات فارسیISIکنفرانسهاژورنالها

Genotyping of Candida albicans Isolates Obtained from Vulvovaginal Candidiasis Patients from Zanjan, Iran, Based on ABC and RPS Typing Systems

محل انتشار: سرطان معده، دوره: 8، شماره: 4
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 62

فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد

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لینک ثابت به این مقاله:
https://civilica.com/doc/1838031

شناسه ملی سند علمی:

JR_CUMM-8-4_002

تاریخ نمایه سازی: 11 آذر 1402

چکیده مقاله:

Background and Purpose: Genotyping of pathogenic microorganisms is important for epidemiological studies and the adoption of appropriate strategies to control infectious diseases. In this regard, the present study aimed to genotype Candida albicans strains isolated from vulvovaginal candidiasis (VVC) patients using combined ABC type (۲۵SrDNA) and repetitive sequence (RPS) typing systems. using combined typing systems of ABC type (۲۵SrDNA) and repetitive sequence (RPS).Materials and Methods: In total, ۱۴۰ patients with VVC were investigated. Vaginal discharges were collected on Sabouraud dextrose agar and identified by CHROMagar. After species identification, a polymerase chain reaction system targeting ۲۵S rDNA as well as ALT repeats in the RPS was designed to determine C. albicans genotypes. The dendrogram was constructed by zero-one matrix data based on the combination of ABC and RPS typing systems. Statistical analysis of data was performed in SPSS software (version ۲۳).Results: In total, ۴۱ (۲۹.۳%) Candida isolates were obtained from ۱۴۰ VVC patients. The most common Candida species that were identified included C. glabrata (۵۶.۱%) and C. albicans (۳۹%). Genotype A۳ with five isolates (۳۱.۲۵%) had the highest frequency, followed by B۲/۳ with three isolates (۱۸.۳%), A۳/۴, C۳/۴, and B۳/۴ with two isolates (۱۲.۵%), and C۲/۳ and C۳ with one isolate (۶.۲۵%), respectively. No significant association was found between the genotypes and antifungal resistance (P<۰.۰۵).Conclusion: The results showed that non-albicans Candida species are more prevalent in VVC patients, compared to C. albicans. The results also indicated that ABC and RPS typings are useful for rapid genotyping and differentiation of C. albicans isolates in regional and small-scale studies.

کلیدواژه ها:

ALT repeat ، candida albicans ، Genotyping ، RPS ، ۲۵S rDNA

نویسندگان

Saeid Amanloo

Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

Masoomeh Zanjani

Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran

Sahar Serajian

Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran

Farzaneh Ahmadi

Department of Biostatistics and Epidemiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

Firoozeh kakavand

Health and Treatment Center of Zanjan, Zanjan University of Medical Sciences, Zanjan, Iran

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  • Friedman DZ, Schwartz IS. Emerging fungal infections: new patients, new ...
  • Tapia CV, Hermosilla G, Fortes P, Alburquenque C, Bucarey S, ...
  • Chong PP, Hadi SR, Lee YL, Phan CL, Tan BC, ...
  • Ying C, Zhang H, Tang Z, Chen H, Gao J, ...
  • Amanloo S, Katiraee F, Didehdar M, Mohammadi J, Alibabaei Z. ...
  • Odds FC, Jacobsen MD. Multilocus sequence typing of pathogenic Candida ...
  • Hattori H, Iwata T, Nakagawa Y, Kawamoto F, Tomita Y, ...
  • Lian C, Zhao J, Zhang Z, Liu W. Genotype of ...
  • Millar BC, Moore JE, Xu J, Walker MJ, Hedderwick S, ...
  • Hattori H, Tanaka R, Chibana H, Kawamoto F, Adachi H, ...
  • Mijiti J, Pu XM, Erfan A, Yaguchi T, Chibana H, ...
  • Iwata T, Hattori H, Chibana H, Mikami Y, Tomita Y, ...
  • McCullough MJ, Clemons KV, Stevens DA. Molecular and phenotypic characterization ...
  • Pfaller MA. Epidemiology of fungal infections: the promise of molecular ...
  • Müller FM, Werner KE, Kasai M, Francesconi A, Chanock SJ, ...
  • Chibana H, Iwaguchi S, Homma M, Chindamporn A, Nakagawa Y, ...
  • Wayne PA. Reference method for broth dilution antifungal susceptibility testing ...
  • Wayne PA. Reference method for broth dilution antifungal susceptibility testing ...
  • Hunter PR. Reproducibility and indices of discriminatory power of microbial ...
  • Pfaller MA, Diekema D. Progress in antifungal susceptibility testing of ...
  • Karahan ZC, Güriz H, Ağırbaşlı H, Balaban N, Göçmen JS, ...
  • Tamai IA, Salehi TZ, Sharifzadeh A, Shokri H, Khosravi AR. ...
  • Tamura M, Watanabe K, Mikami Y, Yazawa K, Nishimura K. ...
  • Xu J, Boyd CM, Livingston E, Meyer W, Madden JF, ...
  • Sampaio P, Gusmao L, Correia A, Alves C, Rodrigues AG, ...
  • Khatib R, Ramanathan J, Riederer KM, DePoister Jr D, Baran ...
  • Lockhart SR, Reed BD, Pierson CL, Soll DR. Most frequent ...
  • Stevens DA, Odds FC, Scherer S. Application of DNA typing ...
  • Mercure S, Montplaisir S, Lernay G. Correlation between the presence ...
  • Millar BC, Xu J, McMullan R, Walker MJ, Hedderwick S, ...
  • Soll DR, Galask R, Schmid J, Hanna C, Mac K, ...
  • Adachi H, Shimizu K, Hattori H, Tanaka R, Chibana H, ...
  • She XD, Wang XJ, Fu MH, Shen YN, Liu WD. ...
  • Fan SR, Bai FY, Liao QP, Liu ZH, Li J, ...
  • Bougnoux ME, Morand S, d'Enfert C. Usefulness of multilocus sequence ...
  • Dodgson AR, Pujol C, Denning DW, Soll DR, Fox AJ. ...
  • Robles JC, Koreen L, Park S, Perlin DS. Multilocus sequence ...
  • Tavanti A, Gow NA, Senesi S, Maiden MC, Odds FC. ...
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