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مقالات فارسیISIکنفرانسهاژورنالها

Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder

محل انتشار: سرطان معده، دوره: 5، شماره: 3
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 47

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لینک ثابت به این مقاله:
https://civilica.com/doc/1838096

شناسه ملی سند علمی:

JR_CUMM-5-3_003

تاریخ نمایه سازی: 11 آذر 1402

چکیده مقاله:

Background and Purpose: Pneumocystis pneumonia (PCP) is one of the most common and life-threatening fungal diseases in patients with human immunodeficiency, treated with immunosuppressive medications. Immunocompetent people can also be a spreading agent for PCP. Regarding this, the aim of the present study was to diagnose and identify Pneumocystis jirovecii in bronchoalveolar lavage (BAL) samples obtained from patients with pulmonary disorder using a molecular method. Materials and Methods: For the purpose of the study, BAL samples (n=۱۳۸) were collected from patients, undergoing bronchoscopy at the different departments of university hospitals affiliated to Mashhad University of Medical Sciences, Mashhad, Iran, during a period of one year (i.e., April ۲۰۱۴ until May ۲۰۱۵). Giemsa staining and molecular identification were carried out for each sample. The samples were also subjected to nested polymerase chain reaction (PCR), sequencing, and genotyping based on mitochondrial ribosomal large subunit (mtLSU rRNA) of P. jirovecii. The phylogenic tree was constructed by MEGA۶ software. Results: The results of direct microscopic examination revealed the presence of P. jirovecii in ۳ (۲.۲%) out of ۱۳۸ samples; in addition, nested PCR and sequencing led to the detection of species in ۱۷ (۱۲.۳%) samples. Out of patients with positive results, ۱۰ (۲۵%) and ۷ (۷.۱%) cases were immunosuppressed and immunocompetent, respectively. The most common clinical symptoms among patients with pneumocystis were fever, dyspnea, and dry cough. In addition, genotypes III and II were the dominant genotypes in our dataset. Conclusion: Nested PCR and sequencing methods showed higher sensitivity and specificity as compared with a direct staining technique. Genotype III was identified as the most dominant type in patients with pulmonary disorder in Mashhad.

کلیدواژه ها:

Bronchoalveolar Lavage (BAL) ، Iran ، Nested-PCR ، Pneumocystis jirovecii ، Pneumocystis pneumonia (PCP)

نویسندگان

Abdolmajid Fata

Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Bahareh Abdollahi

Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Fariba Rezaeitalab

Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Davood Attaran

Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mohsen Najjari

Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Javad Najafzadeh

Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Chazan R. Contemporary clinical diagnostics of respiratory tract infections. Pol ...
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  • Wakefield AE. DNA sequences identical to Pneumocystis carinii f. sp. ...
  • Respaldiza N, Medrano F, Medrano A, Varela J, De La ...
  • Lowe DM, Rangaka MX, Gordon F, James CD, Miller RF. ...
  • Wilson JW, Limper AH, Grys TE, Karre T, Wengenack NL, ...
  • Thomas Jr CF, Limper AH. Pneumocystis pneumonia. New Engl J ...
  • Sowden E, Carmichael AJ. Autoimmune inflammatory disorders, systemic corticosteroids and ...
  • Kovacs JA, Gill VJ, Meshnick S, Masur H. New insights ...
  • Özkoç S, Bayram SD. Investigation of Pneumocystis jirovecii pneumonia and ...
  • Khodadadi H, Mirhendi H, Mohebali M, Kordbacheh P, Zarrinfar H, ...
  • Morris A, Norris KA. Colonization by Pneumocystis jirovecii and its ...
  • Morris A, Wei K, Afshar K, Huang L. Epidemiology and ...
  • Shelhamer JH, Gill VJ, Quinn TC, Crawford SW, Kovacs JA, ...
  • Aboualigalehdari E, Mahmoudabadi AZ, Fatahinia M, Idani E. The prevalence ...
  • Parian M, Fata A, Najafzadeh MJ, Rezaeitalab F. Molecular detection ...
  • Badiee P. Evaluation of pneumocystis jirovecii in normal population. Jundishapur ...
  • Badiee P, Rezapour A, Abbasian A, Foroutan HR, Jafarian H ...
  • Prevalence of colonization and mitochondrial large subunit rRNA mutation of ...
  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. ...
  • Maskell N, Waine D, Lindley A, Pepperell J, Wakefield A, ...
  • Tia T, Putaporntip C, Kosuwin R, Kongpolprom N, Kawkitinarong K, ...
  • Jarboui M, Sellami A, Sellami H, Cheikhrouhou F, Makni F, ...
  • Peters SE, Wakefield AF, Sinclair K, Millard PR, Hopkin JM. ...
  • Beard CB, Carter JL, Keely SP, Huang L, Pieniazek NJ, ...
  • Jarboui MA, Mseddi F, Sellami H, Sellami A, Makni F, ...
  • Gupta R, Mirdha BR, Guleria R, Agarwal SK, Samantaray JC, ...
  • Volpe G, Sbaiz L, Avanzini C, Caramello P, Savoia D. ...
  • Dimonte S, Berrilli F, D’Orazi C, D’Alfonso R, Placco F, ...
  • Khalife S, Aliouat EM, Aliouat-Denis CM, Gantois N, Devos P, ...
  • Medrano FJ, Montes-Cano M, Conde M, De La Horra C, ...
  • Monroy-Vaca EX, de Armas Y, Illnait-Zaragozi MT, Torano G, Diaz ...
  • Nevez G, Jounieaux V, Linas MD, Guyot K, Leophonte P, ...
  • Visconti E, Marinaci S, Zolfo M, Mencarini P, Tamburrini E, ...
  • Helweg-Larsen J, Jensen JS, Dohn B, Benfield TL, Lundgren B. ...
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