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مقالات فارسیISIکنفرانسهاژورنالها

Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

محل انتشار: سرطان معده، دوره: 5، شماره: 2
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 62

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لینک ثابت به این مقاله:
https://civilica.com/doc/1838104

شناسه ملی سند علمی:

JR_CUMM-5-2_004

تاریخ نمایه سازی: 11 آذر 1402

چکیده مقاله:

Background and Purpose: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to discriminate among pathogenic microorganisms in clinical laboratories. The aim of this study was to assess the utility of MALDI-TOF MS in the routine identification of clinical dermatophyte isolates obtained from various geographical regions of Iran. Materials and Methods: A total of ۹۴ isolates, including Trichophyton interdigitale (n=۴۴), T. rubrum (n=۴۰), T. tonsurans (n=۴), Microsporum canis (n=۴), and Epidermophyton floccosum (n=۱), were analyzed in this study. The identity of each isolate was determined by polymerase chani reaction amplification and sequencing of the internal transcribed spacer (ITS) region of nuclear-encoded ribosomal DNA and also MALDI-TOF MS. The obtained data by molecular approach were compared with MALDI-TOF MS. Results: The MALDI-TOF MS led to the identification of ۴۴ (۴۷%) isolates at the species level by generating the spectral score values of ≥ ۲.۰. However, there was not sufficient agreement between the results of the molecular-based ITS identification methods and MALDI-TOF MS in the species identification of ۱۶ (۱۷%) isolates. The Bruker Daltonics database was also not able to identify protein spectra related to ۱۲ isolates (۱۳%), including T. interdigitale (n=۵), T. rubrum (n=۴), M. canis (n=۲), and T. tonsurans (n=۱). Conclusion: According to the results, the utility of MALDI-TOF MS as a routine diagnostic tool for the accurate and reliable identification of dermatophytes can be justified whenever the protein spectra of a large set of worldwide clinical isolates are included in the commercial libraries. In addition, MALDI-TOF MS can be alternatively used to construct an in-house reference database.

کلیدواژه ها:

dermatophytes ، Iran ، ITS phylogeny ، MALDI-TOF MS

نویسندگان

Mohammad Taghi. Hedayati

Mazandaran University of Medical Sciences

Saham Ansari

Shahid Beheshti University of Medical Sciences, Tehran, Iran

Bahram Ahmadi

Bushehr University of Medical Sciences, Bushehr, Iran.

Mojtaba Taghizadeh Armaki

Babol University of Medical Sciences, Babol, Iran

Tahreh Shokohi

Mazandaran University of Medical Sciences, Sari, Iran

Mahdi Abastabar

Mazandaran University of Medical Sciences, Sari, Iran

Halil Er

Faculty of Medicine, University of Akdeniz, Antalya, Turkey.

Betil Özhak

Faculty of Medicine, University of Akdeniz, Antalya, Turkey.

Dilara Öğünç

aculty of Medicine, University of Akdeniz, Antalya, Turkey.

Macit Ilkit

Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana, Turkey

Seyedmojtaba Seyedmousavi

National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

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